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FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site
Abstract A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at −55 with respect to the transcription start site (+1). Binding of FIS a...
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| Published in: | FEMS microbiology letters 2006-04, Vol.257 (1), p.99-105 |
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| Main Authors: | , , , , , |
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| container_end_page | 105 |
| container_issue | 1 |
| container_start_page | 99 |
| container_title | FEMS microbiology letters |
| container_volume | 257 |
| creator | Huo, Yi-Xin Nan, Bei-Yan You, Cong-Hui Tian, Zhe-Xian Kolb, Annie Wang, Yi-Ping |
| description | Abstract
A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at −55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at −55 activate transcriptional initiation of glnAp2, especially when linear templates were used. |
| doi_str_mv | 10.1111/j.1574-6968.2006.00150.x |
| format | article |
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A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at −55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at −55 activate transcriptional initiation of glnAp2, especially when linear templates were used.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2006.00150.x</identifier><identifier>PMID: 16553838</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria ; Bacteriology ; Base Sequence ; Bending ; Bends ; Binding sites ; Biological and medical sciences ; cAMP receptor protein (CRP) ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - metabolism ; E coli ; Enhancer Elements, Genetic ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; factor for inversion stimulation (FIS) ; Factor For Inversion Stimulation Protein - genetics ; Factor For Inversion Stimulation Protein - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genetics ; Glutamate-Ammonia Ligase - chemistry ; Glutamate-Ammonia Ligase - genetics ; Glutamate-Ammonia Ligase - metabolism ; Integration host factor ; integration host factor (IHF) ; Metabolism. Enzymes ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Conformation ; Promoter Regions, Genetic ; Proteins ; protein‐mediated DNA bending ; Transcription initiation ; Transcription, Genetic ; σ54‐RNA polymerase (Eσ54)</subject><ispartof>FEMS microbiology letters, 2006-04, Vol.257 (1), p.99-105</ispartof><rights>2006 Federation of European Microbiological Societies 2006</rights><rights>2006 INIST-CNRS</rights><rights>2006 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17592408$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16553838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huo, Yi-Xin</creatorcontrib><creatorcontrib>Nan, Bei-Yan</creatorcontrib><creatorcontrib>You, Cong-Hui</creatorcontrib><creatorcontrib>Tian, Zhe-Xian</creatorcontrib><creatorcontrib>Kolb, Annie</creatorcontrib><creatorcontrib>Wang, Yi-Ping</creatorcontrib><title>FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at −55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at −55 activate transcriptional initiation of glnAp2, especially when linear templates were used.</description><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Bending</subject><subject>Bends</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>cAMP receptor protein (CRP)</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - metabolism</subject><subject>E coli</subject><subject>Enhancer Elements, Genetic</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>factor for inversion stimulation (FIS)</subject><subject>Factor For Inversion Stimulation Protein - genetics</subject><subject>Factor For Inversion Stimulation Protein - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>Glutamate-Ammonia Ligase - chemistry</subject><subject>Glutamate-Ammonia Ligase - genetics</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Integration host factor</subject><subject>integration host factor (IHF)</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>protein‐mediated DNA bending</subject><subject>Transcription initiation</subject><subject>Transcription, Genetic</subject><subject>σ54‐RNA polymerase (Eσ54)</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp1kc-O0zAQhy0EYsvCKyBLCE4kjO34TxCXatnCSgUOwNlyHYe6SpNgO9B9A848Ik-CQwsrgfBlLM33G9nzIYQJlCSfZ7uScFkVohaqpACiBCAcysMttPjTuI0WwKQqCNTyDN2LcQcAFQVxF50RwTlTTC3QYXX1Hhub_BeTXMSfun45Uux7fBnt1gVvt95gO3T-OQ5D5_DQYoNfvl3ijesbbF2fXHANNgn_-Pad86d4GmMKzuxnMm0dTsH00QY_Jj_0OCYTEo4-ufvoTmu66B6c6jn6uLr8cPG6WL97dXWxXBcDExUUrCYbTgkDJahyjSOKbISUFYNGWk4Fr9umJZQCpaYmDa03RLXU0kpWrSGuZufoyXHuGIbPk4tJ7320rutM74Yp6jxMVjmfwUd_gbthCn1-m6YMBBdESZWphydq2uxdo8fg9yZc698bzcDjE2CiNV2bv299vOEkr2kFM_fiyH31nbu-6YOeDeudnkXqWaSeDetfhvVBr96s8yXH2TE-TON_wsU_YfYTVYWliw</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Huo, Yi-Xin</creator><creator>Nan, Bei-Yan</creator><creator>You, Cong-Hui</creator><creator>Tian, Zhe-Xian</creator><creator>Kolb, Annie</creator><creator>Wang, Yi-Ping</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200604</creationdate><title>FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site</title><author>Huo, Yi-Xin ; Nan, Bei-Yan ; You, Cong-Hui ; Tian, Zhe-Xian ; Kolb, Annie ; Wang, Yi-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o3640-391b521308628ede181b677430d7c52659fdf122022a91d29b18f2c2474fa1e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Bending</topic><topic>Bends</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>cAMP receptor protein (CRP)</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - metabolism</topic><topic>E coli</topic><topic>Enhancer Elements, Genetic</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>factor for inversion stimulation (FIS)</topic><topic>Factor For Inversion Stimulation Protein - genetics</topic><topic>Factor For Inversion Stimulation Protein - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>Glutamate-Ammonia Ligase - chemistry</topic><topic>Glutamate-Ammonia Ligase - genetics</topic><topic>Glutamate-Ammonia Ligase - metabolism</topic><topic>Integration host factor</topic><topic>integration host factor (IHF)</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>protein‐mediated DNA bending</topic><topic>Transcription initiation</topic><topic>Transcription, Genetic</topic><topic>σ54‐RNA polymerase (Eσ54)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huo, Yi-Xin</creatorcontrib><creatorcontrib>Nan, Bei-Yan</creatorcontrib><creatorcontrib>You, Cong-Hui</creatorcontrib><creatorcontrib>Tian, Zhe-Xian</creatorcontrib><creatorcontrib>Kolb, Annie</creatorcontrib><creatorcontrib>Wang, Yi-Ping</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huo, Yi-Xin</au><au>Nan, Bei-Yan</au><au>You, Cong-Hui</au><au>Tian, Zhe-Xian</au><au>Kolb, Annie</au><au>Wang, Yi-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2006-04</date><risdate>2006</risdate><volume>257</volume><issue>1</issue><spage>99</spage><epage>105</epage><pages>99-105</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a σ54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at −55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at −55 activate transcriptional initiation of glnAp2, especially when linear templates were used.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16553838</pmid><doi>10.1111/j.1574-6968.2006.00150.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2006-04, Vol.257 (1), p.99-105 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Bacteria Bacteriology Base Sequence Bending Bends Binding sites Biological and medical sciences cAMP receptor protein (CRP) Deoxyribonucleic acid DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - metabolism E coli Enhancer Elements, Genetic Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism factor for inversion stimulation (FIS) Factor For Inversion Stimulation Protein - genetics Factor For Inversion Stimulation Protein - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetics Glutamate-Ammonia Ligase - chemistry Glutamate-Ammonia Ligase - genetics Glutamate-Ammonia Ligase - metabolism Integration host factor integration host factor (IHF) Metabolism. Enzymes Microbiology Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Proteins protein‐mediated DNA bending Transcription initiation Transcription, Genetic σ54‐RNA polymerase (Eσ54) |
| title | FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at −55, upstream of the transcription start site |
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