Developmental changes in the metabolic protein profiles of wheat endosperm

A combined two‐dimensional gel electrophoresis‐mass spectrometry approach was utilized to identify over 250 proteins of wheat (Triticum aestivum L., cv. Butte 86) starchy endosperm that participate in 13 biochemical processes: ATP interconversion reactions, carbohydrate metabolism, cell division, cy...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-04, Vol.5 (6), p.1594-1611
Main Authors: Vensel, William H., Tanaka, Charlene K., Cai, Nick, Wong, Joshua H., Buchanan, Bob B., Hurkman, William J.
Format: Article
Language:English
Subjects:
Albumins
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Electrophoresis, Gel, Two-Dimensional
Endosperm
Fundamental and applied biological sciences. Psychology
Germination
Globulins
Miscellaneous
Plant Proteins - metabolism
Proteins
Proteome - metabolism
Seeds - metabolism
Seeds - physiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Triticum - metabolism
Triticum - physiology
Triticum aestivum
Wheat
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Summary:A combined two‐dimensional gel electrophoresis‐mass spectrometry approach was utilized to identify over 250 proteins of wheat (Triticum aestivum L., cv. Butte 86) starchy endosperm that participate in 13 biochemical processes: ATP interconversion reactions, carbohydrate metabolism, cell division, cytoskeleton, lipid metabolism, nitrogen metabolism, protein synthesis/assembly, protein turnover, signal transduction, protein storage, stress/defense, transcription/translation, and transport. Endosperm protein populations were compared at early (10 days post‐anthesis, dpa) and late (36 dpa) stages of grain development. Analysis of protein number and spot volume revealed that carbohydrate metabolism, transcription/translation, and protein synthesis/assembly were the principal endosperm functions at 10 dpa followed by nitrogen metabolism, protein turnover, cytoskeleton, cell division, signal transduction, and lipid metabolism. Carbohydrate metabolism and protein synthesis/assembly were also major functions at 36 dpa, but stress/defense and storage were predominant. The results provide insight into biochemical events taking place during wheat grain development and highlight the value of proteomics in characterizing complex biochemical processes. Further, the proteome maps will facilitate future studies addressing the effects of genetic and environmental factors on the development and quality of wheat grain.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200401034