QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.)

• Published in: Theoretical and applied genetics 2005-05, Vol.110 (7), p.1226-1235
• Main Authors: Abdelkhalik, A.F, Shishido, R, Nomura, K, Ikehashi, H
• Format: Article
• Language: English
• Subjects: backcrossing; Biological and medical sciences; chemical constituents of plants; Chlorophyll; Chlorophyll - metabolism; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Crosses, Genetic; Fundamental and applied biological sciences. Psychology; genes; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; Genotype; Heterozygote; incomplete dominance; indica variety; japonica variety; leaves; microsatellite repeats; Minisatellite Repeats - genetics; Oryza - genetics; Oryza sativa; partial-dominance; Phenotype; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - physiology; Pteridophyta, spermatophyta; Quantitative Trait Loci; Rice; senescence; Time Factors; varieties; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-1955-2

In order to identify quantitative trait loci (QTLs) for leaf senescence and related traits in rice (Oryza sativa L.), we developed two backcross populations, indica/japonica// japonica and indica/japonica//indica, using IR36 as the indica parent and Nekken-2 as the japonica parent. The QTLs were mapped using a set of simple sequence-repeat markers (SSRs) in the BC1F1 population. Senescence was characterized in these plants by measuring the leaf chlorophyll content 25 days after flowering (DAF), the reduction in chlorophyll content (the difference between the chlorophyll content at flowering and at 25 DAF), and the number of late-discoloring leaves per panicle at 25 DAF in five plants from each BC1F2 line. These plants were moved into a temperature-controlled growth cabinet at the time of flowering and allowed to mature under identical conditions. Eleven QTLs were detected in the two populations. The major of QTLs for senescence were found on the short arm of chromosome 6 and on the long arm of chromosome 9. Of these, one QTL on chromosome 6 and two on chromosome 9 were verified by confirming the effects of the genotypes on the phenotypes of the BC1F3 lines. The japonica parent was found to contribute to late senescence at all but one QTL. Based on a comparison of the effects of heterozygotes and homozygotes on the phenotypic values of each QTL genotype, we concluded that the differential senescence observed in the indica-japonica hybrid was not due to over-dominance; rather, it was the result of partial-dominance genes that were donated from either of the parents.