Lipid Peroxidation and Coupled Vitamin Oxidation in Simulated and Human Gastric Fluid Inhibited by Dietary Polyphenols:  Health Implications

• Published in: Journal of agricultural and food chemistry 2005-05, Vol.53 (9), p.3397-3402
• Main Authors: Gorelik, Shlomit, Lapidot, Tair, Shaham, Inbal, Granit, Rina, Ligumsky, Moshe, Kohen, Ron, Kanner, Joseph
• Format: Article
• Language: English
• Subjects: acidity; Animals; Antioxidants - administration & dosage; ascorbic acid; beta Carotene - chemistry; beta-carotene; Biological and medical sciences; catechin; Catechin - pharmacology; Diet; Feeding. Feeding behavior; Flavonoids - pharmacology; Fundamental and applied biological sciences. Psychology; gastric juice; Gastric Juice - chemistry; Health Promotion; Hot Temperature; Humans; Hydrogen-Ion Concentration; in vitro digestion; lipid peroxidation; Lipid Peroxidation - drug effects; Meat - analysis; Models, Biological; oxidation; Oxidation-Reduction; Phenols - pharmacology; Polyphenols; red wines; Soybean Oil - chemistry; turkey meat; Vertebrates: anatomy and physiology, studies on body, several organs or systems; vitamin E; Vitamins - chemistry; Wine - analysis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf040401o

The Western diet contains large quantities of oxidized lipids, because a large proportion of the food in the diet is consumed in a fried, heated, processed, or stored form. We investigated the reaction that could occur in the acidic pH of the stomach and accelerate the generation of lipid hydroperoxides and cooxidation of dietary vitamins. To estimate the oxygen content in the stomach after food consumption, oxygen released from masticated bread (20 g) into deoxygenated water (100 mL) was measured. Under these conditions, the oxygen concentration rose by 250 μM and reached a full oxygen saturation. The present study demonstrated that heated red meat homogenized in human gastric fluid, at pH 3.0, generated hydroperoxides and malondialdehyde. The cross-reaction between free radicals produced during this reaction cooxidized vitamin E, β-carotene, and vitamin C. Both lipid peroxidation and cooxidation of vitamin E and β-carotene were inhibited at pH 3.0 by red wine polyphenols. Ascorbic acid (44 mg) at a concentration that represented the amount that could be ingested during a meal inhibited lipid peroxidation only slightly. Red wine polyphenols failed to prevent ascorbic acid oxidation significantly but, in conjunction with ascorbic acid, did inhibit lipid peroxidation. In the presence of catechin, a well-known polyphenol found in red wine, ascorbic acid at pH 3.0 works in a synergistic manner preventing lipid peroxidation and β-carotene cooxidation. The present data may explain the major benefits to our health and the crucial role of consuming food products rich in dietary antioxidants such as fruits, vegetables, red wines, or green tea during the meal. Keywords: Lipid peroxidation; vitamins E and C; β-carotene; human gastric fluid; dietary polyphenols antioxidants; health