Pterostilbene, a New Agonist for the Peroxisome Proliferator-Activated Receptor α-Isoform, Lowers Plasma Lipoproteins and Cholesterol in Hypercholesterolemic Hamsters

• Published in: Journal of agricultural and food chemistry 2005-05, Vol.53 (9), p.3403-3407
• Main Authors: Rimando, Agnes M, Nagmani, Rangaswamy, Feller, Dennis R, Yokoyama, Wallace
• Format: Article
• Language: English
• Subjects: Acyl-CoA Oxidase - genetics; agonists; Animals; Biological and medical sciences; blood plasma; cholesterol; Cholesterol - blood; Cricetinae; gene expression; gene induction; General and cellular metabolism. Vitamins; grapes; hamsters; high density lipoprotein; hypercholesterolemia; Hypercholesterolemia - blood; Hypercholesterolemia - drug therapy; lipemic effect; Lipoproteins - blood; Liver Neoplasms, Experimental; low density lipoprotein; luciferase; Male; Medical sciences; Mesocricetus; Pharmacology. Drug treatments; Phenols - administration & dosage; piceatannol; PPAR alpha - agonists; PPAR alpha - genetics; pterostilbene; Rats; receptors; Recombinant Fusion Proteins; reporter genes; Response Elements - genetics; resveratrol; resveratrol trimethyl ether; stilbenes; Stilbenes - administration & dosage; transcription factors; Transfection; Tumor Cells, Cultured
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0580364

Resveratrol, a stilbenoid antioxidant found in grapes, wine, peanuts and other berries, has been reported to have hypolipidemic properties. We investigated whether resveratrol and its three analogues (pterostilbene, piceatannol, and resveratrol trimethyl ether) would activate the peroxisome proliferator-activated receptor α (PPARα) isoform. This nuclear receptor is proposed to mediate the activity of lipid-lowering drugs such as the fibrates. The four stilbenes were evaluated at 1, 10, 100, and 300 μM along with ciprofibrate (positive control), for the activation of endogenous PPARα in H4IIEC3 cells. Cells were transfected with a peroxisome proliferator response element-AB (rat fatty acyl CoA β-oxidase response element)luciferase gene reporter construct. Pterostilbene demonstrated the highest induction of PPARα showing 8- and 14-fold increases in luciferase activity at 100 and 300 μM, respectively, relative to the control. The maximal luciferase activity responses to pterostilbene were higher than those obtained with the hypolipidemic drug, ciprofibrate (33910 and 19460 relative luciferase units, respectively), at 100 μM. Hypercholesterolemic hamsters fed with pterostilbene at 25 ppm of the diet showed 29% lower plasma low density lipoprotein (LDL) cholesterol, 7% higher plasma high density lipoprotein (HDL) cholesterol, and 14% lower plasma glucose as compared to the control group. The LDL/HDL ratio was also statistically significantly lower for pterostilbene, as compared to results for the control animals, at this diet concentration. Results from in vitro studies showed that pterostilbene acts as a PPARα agonist and may be a more effective PPARα agonist and hypolipidemic agent than resveratrol. In vivo studies demonstrate that pterostilbene possesses lipid and glucose lowering effects. Keywords: Pterostilbene; PPARα; cholesterol; lipoprotein