Anterograde axonal transport of BDNF and NT-3 by retinal ganglion cells: Roles of neurotrophin receptors

Retinal ganglion cells (RGCs) transport exogenous neurotrophins anterogradely to the midbrain tectum/superior colliculus with significant downstream effects. We determined contributions of neurotrophin receptors for anterograde transport of intraocularly injected radiolabeled neurotrophins. In adult...

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Published in:Molecular and cellular neuroscience 2005-05, Vol.29 (1), p.11-25
Main Authors: Butowt, Rafal, von Bartheld, Christopher S.
Format: Article
Language:English
Subjects:
Animals
Antibodies - pharmacology
Axonal Transport - physiology
Brain-Derived Neurotrophic Factor - pharmacokinetics
Chick Embryo
Chickens
Iodine Radioisotopes
Mice
Mice, Inbred C57BL
Mice, Knockout
Neurotrophin 3 - pharmacokinetics
Oligonucleotides, Antisense - pharmacology
Rats
Rats, Wistar
Receptor, Nerve Growth Factor
Receptor, trkB - immunology
Receptors, Nerve Growth Factor - genetics
Receptors, Nerve Growth Factor - immunology
Receptors, Nerve Growth Factor - metabolism
Retinal Ganglion Cells - metabolism
Superior Colliculi - cytology
Superior Colliculi - metabolism
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Summary:Retinal ganglion cells (RGCs) transport exogenous neurotrophins anterogradely to the midbrain tectum/superior colliculus with significant downstream effects. We determined contributions of neurotrophin receptors for anterograde transport of intraocularly injected radiolabeled neurotrophins. In adult rodents, anterograde transport of brain-derived neurotrophic factor (BDNF) was receptor-mediated, and transport of exogenous BDNF and neurotrophin-3 (NT-3) was more efficient, per RGC, in rodents than chicks. RT-PCR and Western blot analysis of purified murine RGCs showed that adult RGCs express the p75 receptor. Anterograde transport of BDNF or NT-3 was not diminished in p75 knock-out mice (with unaltered final numbers of RGCs), but BDNF transport was substantially reduced by co-injected trkB antibodies. In chick embryos, however, p75 antisense or co-injected p75 antibodies significantly attenuated anterograde transport of NT-3 by RGCs. Thus, neither BDNF nor NT-3 utilizes p75 for anterograde transport in adult rodent RGCs, while anterograde NT-3 transport requires the p75 receptor in embryonic chicken RGCs.
ISSN:1044-7431
1095-9327
DOI:10.1016/j.mcn.2005.02.004