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Silkworm pathogenic bacteria infection model for identification of novel virulence genes
Summary Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for thei...
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| Published in: | Molecular microbiology 2005-05, Vol.56 (4), p.934-944 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5716-76063db4d707307d337f85ba751cf3be9d718b52dcdf24bd3003480fdd609fc93 |
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| cites | cdi_FETCH-LOGICAL-c5716-76063db4d707307d337f85ba751cf3be9d718b52dcdf24bd3003480fdd609fc93 |
| container_end_page | 944 |
| container_issue | 4 |
| container_start_page | 934 |
| container_title | Molecular microbiology |
| container_volume | 56 |
| creator | Kaito, Chikara Kurokawa, Kenji Matsumoto, Yasuhiko Terao, Yutaka Kawabata, Shigetada Hamada, Shigeyuki Sekimizu, Kazuhisa |
| description | Summary
Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for their virulence in silkworms. Of these 100 genes, three genes named cvfA, cvfB, and cvfC were required for full virulence of S. aureus in silkworms. Haemolysin production was decreased in these mutants. The cvfA and cvfC mutants also had attenuated virulence in mice. S. pyogenes cvfA‐disrupted mutants produced less exotoxin and had attenuated virulence in both silkworms and mice. These results indicate that the silkworm‐infection model is useful for identifying bacterial virulence genes. |
| doi_str_mv | 10.1111/j.1365-2958.2005.04596.x |
| format | article |
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Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for their virulence in silkworms. Of these 100 genes, three genes named cvfA, cvfB, and cvfC were required for full virulence of S. aureus in silkworms. Haemolysin production was decreased in these mutants. The cvfA and cvfC mutants also had attenuated virulence in mice. S. pyogenes cvfA‐disrupted mutants produced less exotoxin and had attenuated virulence in both silkworms and mice. These results indicate that the silkworm‐infection model is useful for identifying bacterial virulence genes.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2005.04596.x</identifier><identifier>PMID: 15853881</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Bacteria ; Bacteriology ; Biological and medical sciences ; Bombyx - microbiology ; Bombyx mori ; Disease Models, Animal ; Female ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Bacterial ; Hemolysin Proteins - metabolism ; Infectious diseases ; Mice ; Microbiology ; Mutation ; Open Reading Frames ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Pathogens ; Protein Structure, Tertiary ; Staphylococcal Infections - microbiology ; Staphylococcus - genetics ; Staphylococcus - metabolism ; Staphylococcus - pathogenicity ; Staphylococcus aureus ; Streptococcus pyogenes ; Streptococcus pyogenes - genetics ; Streptococcus pyogenes - pathogenicity ; Survival Rate ; Virulence - genetics ; Worms</subject><ispartof>Molecular microbiology, 2005-05, Vol.56 (4), p.934-944</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Blackwell Publishing May 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5716-76063db4d707307d337f85ba751cf3be9d718b52dcdf24bd3003480fdd609fc93</citedby><cites>FETCH-LOGICAL-c5716-76063db4d707307d337f85ba751cf3be9d718b52dcdf24bd3003480fdd609fc93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16735538$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15853881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaito, Chikara</creatorcontrib><creatorcontrib>Kurokawa, Kenji</creatorcontrib><creatorcontrib>Matsumoto, Yasuhiko</creatorcontrib><creatorcontrib>Terao, Yutaka</creatorcontrib><creatorcontrib>Kawabata, Shigetada</creatorcontrib><creatorcontrib>Hamada, Shigeyuki</creatorcontrib><creatorcontrib>Sekimizu, Kazuhisa</creatorcontrib><title>Silkworm pathogenic bacteria infection model for identification of novel virulence genes</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for their virulence in silkworms. Of these 100 genes, three genes named cvfA, cvfB, and cvfC were required for full virulence of S. aureus in silkworms. Haemolysin production was decreased in these mutants. The cvfA and cvfC mutants also had attenuated virulence in mice. S. pyogenes cvfA‐disrupted mutants produced less exotoxin and had attenuated virulence in both silkworms and mice. These results indicate that the silkworm‐infection model is useful for identifying bacterial virulence genes.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bombyx - microbiology</subject><subject>Bombyx mori</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Hemolysin Proteins - metabolism</subject><subject>Infectious diseases</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Open Reading Frames</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Pathogens</subject><subject>Protein Structure, Tertiary</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - metabolism</subject><subject>Staphylococcus - pathogenicity</subject><subject>Staphylococcus aureus</subject><subject>Streptococcus pyogenes</subject><subject>Streptococcus pyogenes - genetics</subject><subject>Streptococcus pyogenes - pathogenicity</subject><subject>Survival Rate</subject><subject>Virulence - genetics</subject><subject>Worms</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkUtrFTEYhoNY7LH6FyQIuptpLieXWbiQUmuhpYsqdBcyuWiOM8kxmenl3zfTc7DgRrNJ4H2-ly88AECMWlzP8abFlLOGdEy2BCHWojXreHv_Aqz-BC_BCnUMNVSSm0PwupQNQpgiTl-BQ8wko1LiFbi5DsOvu5RHuNXTz_TDxWBgr83kctAwRO_MFFKEY7JugD5lGKyLU_DB6KcgeRjTbc1uQ54HF42DtcSVN-DA66G4t_v7CHz_cvrt5GtzcXV2fvL5ojFMYN4IXjey_doKJCgSllLhJeu1YNh42rvOCix7Rqyxnqx7SxGia4m8tRx13nT0CHzc9W5z-j27MqkxFOOGQUeX5qK4EB3CnP8TxIJRgiWp4Pu_wE2ac6yfULjjjAhBWIXkDjI5lZKdV9scRp0fFEZqcaQ2alGhFhVqcaSeHKn7Ovpu3z_3o7PPg3spFfiwB3QxevBZRxPKM8cFZZWs3KcddxcG9_DfC6jLy_PlRR8Bml2s7Q</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Kaito, Chikara</creator><creator>Kurokawa, Kenji</creator><creator>Matsumoto, Yasuhiko</creator><creator>Terao, Yutaka</creator><creator>Kawabata, Shigetada</creator><creator>Hamada, Shigeyuki</creator><creator>Sekimizu, Kazuhisa</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Silkworm pathogenic bacteria infection model for identification of novel virulence genes</title><author>Kaito, Chikara ; Kurokawa, Kenji ; Matsumoto, Yasuhiko ; Terao, Yutaka ; Kawabata, Shigetada ; Hamada, Shigeyuki ; Sekimizu, Kazuhisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5716-76063db4d707307d337f85ba751cf3be9d718b52dcdf24bd3003480fdd609fc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bombyx - microbiology</topic><topic>Bombyx mori</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Hemolysin Proteins - metabolism</topic><topic>Infectious diseases</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Open Reading Frames</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Pathogens</topic><topic>Protein Structure, Tertiary</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - metabolism</topic><topic>Staphylococcus - pathogenicity</topic><topic>Staphylococcus aureus</topic><topic>Streptococcus pyogenes</topic><topic>Streptococcus pyogenes - genetics</topic><topic>Streptococcus pyogenes - pathogenicity</topic><topic>Survival Rate</topic><topic>Virulence - genetics</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaito, Chikara</creatorcontrib><creatorcontrib>Kurokawa, Kenji</creatorcontrib><creatorcontrib>Matsumoto, Yasuhiko</creatorcontrib><creatorcontrib>Terao, Yutaka</creatorcontrib><creatorcontrib>Kawabata, Shigetada</creatorcontrib><creatorcontrib>Hamada, Shigeyuki</creatorcontrib><creatorcontrib>Sekimizu, Kazuhisa</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaito, Chikara</au><au>Kurokawa, Kenji</au><au>Matsumoto, Yasuhiko</au><au>Terao, Yutaka</au><au>Kawabata, Shigetada</au><au>Hamada, Shigeyuki</au><au>Sekimizu, Kazuhisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silkworm pathogenic bacteria infection model for identification of novel virulence genes</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2005-05</date><risdate>2005</risdate><volume>56</volume><issue>4</issue><spage>934</spage><epage>944</epage><pages>934-944</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for their virulence in silkworms. Of these 100 genes, three genes named cvfA, cvfB, and cvfC were required for full virulence of S. aureus in silkworms. Haemolysin production was decreased in these mutants. The cvfA and cvfC mutants also had attenuated virulence in mice. S. pyogenes cvfA‐disrupted mutants produced less exotoxin and had attenuated virulence in both silkworms and mice. These results indicate that the silkworm‐infection model is useful for identifying bacterial virulence genes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15853881</pmid><doi>10.1111/j.1365-2958.2005.04596.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular microbiology, 2005-05, Vol.56 (4), p.934-944 |
| issn | 0950-382X 1365-2958 |
| language | eng |
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| source | Wiley |
| subjects | Animals Bacteria Bacteriology Biological and medical sciences Bombyx - microbiology Bombyx mori Disease Models, Animal Female Fundamental and applied biological sciences. Psychology Genes Genes, Bacterial Hemolysin Proteins - metabolism Infectious diseases Mice Microbiology Mutation Open Reading Frames Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Pathogens Protein Structure, Tertiary Staphylococcal Infections - microbiology Staphylococcus - genetics Staphylococcus - metabolism Staphylococcus - pathogenicity Staphylococcus aureus Streptococcus pyogenes Streptococcus pyogenes - genetics Streptococcus pyogenes - pathogenicity Survival Rate Virulence - genetics Worms |
| title | Silkworm pathogenic bacteria infection model for identification of novel virulence genes |
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