Localization of equilibrative nucleoside transporters in the rat brain

Adenosine is a recognized inhibitory neuromodulator and neuroprotective agent in the central nervous system. It is produced both intra- and extracellularly and transported across the cell membrane. Nucleoside transporters thus have a major impact on the extracellular adenosine levels, and consequent...

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Bibliographic Details
Published in:Journal of chemical neuroanatomy 2006-04, Vol.31 (3), p.162-168
Main Authors: Alanko, Lauri, Porkka-Heiskanen, Tarja, Soinila, Seppo
Format: Article
Language:English
Subjects:
Adenosine
Adenosine metabolism
Animals
Antibody Specificity
Blotting, Western
Brain - metabolism
Epilepsy
Equilibrative Nucleoside Transport Proteins - immunology
Equilibrative Nucleoside Transport Proteins - metabolism
Immunohistochemistry
Ischemia
Male
Neuroglia - metabolism
Neurons - metabolism
Nucleoside transport
Rat brain
Rats
Rats, Wistar
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Summary:Adenosine is a recognized inhibitory neuromodulator and neuroprotective agent in the central nervous system. It is produced both intra- and extracellularly and transported across the cell membrane. Nucleoside transporters thus have a major impact on the extracellular adenosine levels, and consequently adenosine signalling. We have raised and characterized polyclonal antibodies against both the equilibrative nucleoside transporters 1 and 2, and report for the first time their distribution in rat brain at the cellular level. Double staining studies were performed to assess the localization of the transporters in neural and glial cells. Both transporters were present in practically all neurons. Some astrocytes showed equilibrative nucleoside transporter 1 staining, while equilibrative nucleoside transporter 2 staining on astrocytes was observed only sporadically.
ISSN:0891-0618
1873-6300
DOI:10.1016/j.jchemneu.2005.12.001