Development and gene expression profiling of a metastatic variant of the human breast cancer MDA-MB-435 cells

We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when tran...

Full description

Saved in:
Bibliographic Details
Published in:Cancer biology & therapy 2005-02, Vol.4 (2), p.168-174
Main Authors: Bandyopadhyay, Abhik, Elkahloun, Abdel, Baysa, Sherrie Joy, Wang, Long, Sun, Lu-Zhe
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - metabolism
Bone Neoplasms - genetics
Bone Neoplasms - secondary
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Female
Gene Expression
Gene Expression Profiling
Green Fluorescent Proteins - genetics
Humans
Lung Neoplasms - genetics
Lung Neoplasms - secondary
Mice
Mice, Nude
Neoplasm Proteins - genetics
Neoplasms, Experimental
Oligonucleotide Array Sequence Analysis
Transplantation, Heterologous
Tumor Cells, Cultured
Tumor Stem Cell Assay
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a model system of late stage metastatic progression by isolating a highly malignant variant of human breast cancer cells from the parental MDA-MB-435 cell line. These cells, isolated from early lung metastasis, displayed increased anchorage independent growth in vitro and when transplanted ortho-topically into nude mice showed accelerated tumor growth rate and early lung spontaneous metastasis when compared to its parental counterpart. These cells, designated as MDA-MB-435-F-L, also showed intense wide spread early skeletal metastasis in vertebrae, mandible, femur, tibia and skull as detected by fluorescence imaging in an experimental bone metastasis model. Gene expression profiles from cDNA microarray showed up or downregulation of the expression of several significant genes regulating angiogenesis, apoptosis, ECM remodeling and metastasis in the MDA-MB-435-F-L cells in comparison to the parental cells. Among the up or downregulated genes, some have also been implicated in the survival of breast cancer patients. As such, the candidate genes selected in this breast cancer progression model system may serve as biomarkers of metastatic progression and also as potential tumor targets for breast cancer therapy.
ISSN:1538-4047