Telomere configuration influences the choice of telomere maintenance pathways

Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of telomerase. In yeast cells that lack telomerase, telomeres are maintained by alternative type I and type II recombination mechanisms. Previous studies identified several proteins to con...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-05, Vol.343 (2), p.459-466
Main Authors: Wen, Wan-Ying, Tsai, Hung-Ji, Lin, Chuan-Chuan, Tseng, Shun-Fu, Wong, Chui-Wei, Teng, Shu-Chun
Format: Article
Language:English
Subjects:
Base Sequence
Cancer
DNA recombination
DNA replication
DNA Replication - genetics
Molecular Sequence Data
Recombination, Genetic - genetics
Saccharomycetales - genetics
Signal Transduction - genetics
Structure-Activity Relationship
Telomerase
Telomere
Telomere - genetics
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Summary:Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of telomerase. In yeast cells that lack telomerase, telomeres are maintained by alternative type I and type II recombination mechanisms. Previous studies identified several proteins to control the choice between two types of recombinations. Here, we demonstrate that configuration of telomeres also plays a role to determine the fate of telomere replication in progeny. When diploid yeasts from mating equip with a specific type of telomeric structure in their genomes, they prefer to maintain this type of telomere replication in their descendants. While inherited telomere structure is easier to be utilized in progeny at the beginning stage, the telomeres in type I diploids can gradually switch to the type II cells in liquid culture. Importantly, the TLC1/ tlc1 yeast cells develop type II survivors suggesting that haploid insufficiency of telomerase RNA component, which is similar to a type of dyskeratosis congenital in human. Altogether, our results suggest that both protein factors and substrate availability contribute to the choice among telomere replication pathways in yeast.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.03.011