Simultaneously Amplified Electrochemical and Surface Plasmon Optical Detection of DNA Hybridization Based on Ferrocene−Streptavidin Conjugates

A sensitive method based on ferrocene−streptavidin (Fc−Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc−Stv to the biotinylated complemen...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-05, Vol.77 (9), p.2756-2761
Main Authors: Liu, Jianyun, Tian, Shengjun, Tiefenauer, Louis, Nielsen, Peter Eigil, Knoll, Wolfgang
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemical compounds
Chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
Electric currents
Electrochemical methods
Electrochemistry - methods
Exact sciences and technology
Ferrous Compounds - chemistry
General, instrumentation
Hybridization
Metallocenes
Nucleic Acid Hybridization
Peptide Nucleic Acids - chemistry
Sensors
Streptavidin - chemistry
Surface Plasmon Resonance - methods
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Summary:A sensitive method based on ferrocene−streptavidin (Fc−Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc−Stv to the biotinylated complementary target DNA not only amplified the surface plasmon resonance signal but also enhanced the electrochemical signal due to the many Fc markers per Stv. The ferrocene redox peak current increased with the increase of the target DNA concentration. Consequently, the amount of hybridized target DNA can be estimated by cyclic voltammetry and chronocoulometry. The detection limit of this DNA sensor is 10 pM (2 fmol, with signal to noise > 3). This sensor was also shown to have high selectivity (at the single-base mismatch level) and good reproducibility.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac048088c