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Simultaneously Amplified Electrochemical and Surface Plasmon Optical Detection of DNA Hybridization Based on Ferrocene−Streptavidin Conjugates

A sensitive method based on ferrocene−streptavidin (Fc−Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc−Stv to the biotinylated complemen...

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Published in:	Analytical chemistry (Washington) 2005-05, Vol.77 (9), p.2756-2761
Main Authors:	Liu, Jianyun, Tian, Shengjun, Tiefenauer, Louis, Nielsen, Peter Eigil, Knoll, Wolfgang
Format:	Article
Language:	English
Subjects:	Analytical chemistry Chemical compounds Chemistry Deoxyribonucleic acid DNA DNA - chemistry Electric currents Electrochemical methods Electrochemistry - methods Exact sciences and technology Ferrous Compounds - chemistry General, instrumentation Hybridization Metallocenes Nucleic Acid Hybridization Peptide Nucleic Acids - chemistry Sensors Streptavidin - chemistry Surface Plasmon Resonance - methods
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creator	Liu, Jianyun Tian, Shengjun Tiefenauer, Louis Nielsen, Peter Eigil Knoll, Wolfgang
description	A sensitive method based on ferrocene−streptavidin (Fc−Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc−Stv to the biotinylated complementary target DNA not only amplified the surface plasmon resonance signal but also enhanced the electrochemical signal due to the many Fc markers per Stv. The ferrocene redox peak current increased with the increase of the target DNA concentration. Consequently, the amount of hybridized target DNA can be estimated by cyclic voltammetry and chronocoulometry. The detection limit of this DNA sensor is 10 pM (2 fmol, with signal to noise > 3). This sensor was also shown to have high selectivity (at the single-base mismatch level) and good reproducibility.
doi_str_mv	10.1021/ac048088c
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Chem</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>77</volume><issue>9</issue><spage>2756</spage><epage>2761</epage><pages>2756-2761</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A sensitive method based on ferrocene−streptavidin (Fc−Stv) conjugates for the simultaneously amplified electrochemical and surface plasmon optical detection of DNA target hybridization to peptide nucleic acid-modified gold surfaces is reported. The attachment of Fc−Stv to the biotinylated complementary target DNA not only amplified the surface plasmon resonance signal but also enhanced the electrochemical signal due to the many Fc markers per Stv. The ferrocene redox peak current increased with the increase of the target DNA concentration. Consequently, the amount of hybridized target DNA can be estimated by cyclic voltammetry and chronocoulometry. The detection limit of this DNA sensor is 10 pM (2 fmol, with signal to noise > 3). 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source	American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects	Analytical chemistry Chemical compounds Chemistry Deoxyribonucleic acid DNA DNA - chemistry Electric currents Electrochemical methods Electrochemistry - methods Exact sciences and technology Ferrous Compounds - chemistry General, instrumentation Hybridization Metallocenes Nucleic Acid Hybridization Peptide Nucleic Acids - chemistry Sensors Streptavidin - chemistry Surface Plasmon Resonance - methods
title	Simultaneously Amplified Electrochemical and Surface Plasmon Optical Detection of DNA Hybridization Based on Ferrocene−Streptavidin Conjugates
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