Effect of synthetic colloids on major histocompatibility complex class II expression

• Published in: Journal of clinical anesthesia 2006-03, Vol.18 (2), p.96-101
• Main Authors: Haeberle, Helene A., Nohé, Boris, Eltzschig, Holger K., Unertl, Klaus, Dieterich, Hans-Juergen
• Format: Article
• Language: English
• Subjects: Anesthesia; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; Clinical trials; Colloids - pharmacology; Dextrans - pharmacology; Dose-Response Relationship, Drug; Flow Cytometry; Fluorescent Antibody Technique; Gelatin - pharmacology; Gene Expression - drug effects; Genes, MHC Class II - genetics; HLA Antigens - biosynthesis; Human; Human leukocyte antigen; Humans; Hydroxyethyl Starch Derivatives - pharmacology; In Vitro Techniques; Interferon-gamma - pharmacology; Lymphocytes; Lymphocytes - drug effects; Lymphocytes - metabolism; Major histocompatibility complex; Medical sciences; Monocytes - drug effects; Monocytes - metabolism; Osmotic Pressure; Plasma; Plasma Substitutes - pharmacology; Proteins; Recombinant Proteins; Serum Albumin - metabolism; Statistical analysis; Stimulation, Chemical; Studies; Synthetic colloids
• ISSN: 0952-8180; 1873-4529
• DOI: 10.1016/j.jclinane.2005.06.010

Synthetic colloids are used for perioperative fluid management. We hypothesized that their use may be associated with changes in major histocompatibility complex (MHC) class II expression. This could affect patients' morbidity and mortality during clinical intervention. University research laboratory. Whole blood samples from healthy volunteers. Whole blood samples from healthy volunteers (n = 6) were incubated with different concentrations of hydroxyethyl starch (HES) from maize and potato (pHES), dextran, and polygelin (gelatine) for 24 hours with or without 100 U/mL human interferon γ (IFN- γ; stimulus for MHC class II expression). The expression of human leukocyte antigen (HLA): HLA-DR, HLA-DQ, and HLA-DP was detected simultaneously by a fluoresceinisothiocyanate (FITC)-labeled antibody and analyzed by flow cytometry on lymphocytes and monocytes. Hydroxyethyl starch, pHES, and dextran induced a significant increase in HLA expression. The induction of MHC class II was independent of the structure (50 mg/mL: control, 8.7±1.4%; HES, 28 ± 9.7%; pHES, 29.8 ± 11.7%; and dextran, 50.2 ± 8.1%). In contrast, polygelin increased HLA expression only at the highest concentration of gelatine (5 mg/mL, 7.8 ± 1%; 50 mg/mL, 7.6 ± 0.8%; 100 mg/mL, 7.3 ± 1%; 200 mg/mL,16.2 ± 2.3%). The addition of IFN- γ decreased HLA expression in presence of highest concentration of HES and dextran. In an ex vivo laboratory setting, we demonstrate that high concentrations of plasma expanders are associated with increased HLA expression on lymphocytes and monocytes. However, further in vivo studies are necessary to demonstrate the clinical significance of this observation.