Influence of Chromatin and Single Strand Binding Proteins on the Activity of an Archaeal MCM

The mini-chromosome maintenance (MCM) complex is the presumptive replicative helicase in archaea and eukaryotes. In archaea, the MCM is a homo-multimer, in eukaryotes a heterohexamer composed of six related subunits, MCM 2–7. Biochemical studies using naked DNA templates have revealed that archaeal...

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Bibliographic Details
Published in:Journal of molecular biology 2006-04, Vol.357 (5), p.1345-1350
Main Authors: Marsh, Victoria L., McGeoch, Adam T., Bell, Stephen D.
Format: Article
Language:English
Subjects:
Acetylation
Archaea
Archaeal Proteins - metabolism
chromatin
Chromatin - metabolism
DNA Helicases - metabolism
DNA replication
DNA-Binding Proteins - metabolism
MCM
Multiprotein Complexes - metabolism
Nucleic Acid Denaturation
Sulfolobus solfataricus
Sulfolobus solfataricus - genetics
Sulfolobus solfataricus - metabolism
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Summary:The mini-chromosome maintenance (MCM) complex is the presumptive replicative helicase in archaea and eukaryotes. In archaea, the MCM is a homo-multimer, in eukaryotes a heterohexamer composed of six related subunits, MCM 2–7. Biochemical studies using naked DNA templates have revealed that archaeal MCMs and a sub-complex of eukaryotic MCM 4, 6 and 7 have 3′ to 5′ helicase activity. Here, we investigate the influence of the major chromatin proteins, Alba and Sul7d, of Sulfolobus solfataricus (Sso) on the ability of the MCM complex to melt partial duplex DNA substrates. In addition, we test the effect of Sso SSB on MCM activity. We reveal that Alba represents a formidable barrier to MCM activity and further demonstrate that acetylation of Alba alleviates repression of MCM activity.
ISSN:0022-2836
1089-8638
DOI:10.1016/j.jmb.2006.01.074