Gene silencing in mosquito salivary glands by RNAi

Salivary glands are the ultimate site of development in the insect of mosquito born pathogens such as Plasmodium. Mosquito salivary glands also secrete components involved in anti-haemostatic activities and allergic reactions. We investigated the feasibility of RNAi as a tool for functional analysis...

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Bibliographic Details
Published in:FEBS letters 2006-04, Vol.580 (8), p.1988-1992
Main Authors: Boisson, Bertrand, Jacques, Jean Claude, Choumet, Valérie, Martin, Estelle, Xu, Jiannong, Vernick, Ken, Bourgouin, Catherine
Format: Article
Language:English
Subjects:
Animals
Anopheles
Anopheles - anatomy & histology
Anopheles - genetics
Anopheles gambiae
Apyrase
Apyrase - genetics
Apyrase - metabolism
Male
Plasmodium
Platelet Aggregation
Rabbits
Recombinant Fusion Proteins - metabolism
RNA Interference
RNA, Double-Stranded - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - genetics
RNAi
Salivary gland
Salivary Glands - metabolism
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Summary:Salivary glands are the ultimate site of development in the insect of mosquito born pathogens such as Plasmodium. Mosquito salivary glands also secrete components involved in anti-haemostatic activities and allergic reactions. We investigated the feasibility of RNAi as a tool for functional analysis of genes expressed in Anopheles gambiae salivary glands. We show that specific gene silencing in salivary glands requires the use of large amounts of dsRNA, condition that differs from those for efficient RNAi in other mosquito tissues. Using this protocol, we demonstrated the role of AgApy, which encodes an apyrase, in the probing behaviour of An. gambiae.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.02.069