Immunogenicity of two Echinococcus granulosus antigens EgA31 and EgTrp in mice

Here, we investigate in mice the immunogenicity of two antigens EgA31 and EgTrp which are expressed by the larval stage of Echinococcus granulosus. These recombinant proteins were used alone or as a mixture (EgA31-EgTrp) to immunize BALB/c mice. By flow cytometry, we have shown that the ratio CD4+/C...

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Bibliographic Details
Published in:Parasitology research (1987) 2005-05, Vol.96 (2), p.113-120
Main Authors: FRAIZE, M, SARCIRON, M. E, AZZOUZ, S, ISSAADI, N, BOSQUET, G, PETAVY, A. F
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - blood
Antigens, Helminth - administration & dosage
Antigens, Helminth - genetics
Antigens, Helminth - immunology
Biological and medical sciences
CD4-CD8 Ratio
Cytokines - metabolism
Echinococcosis - mortality
Echinococcosis - prevention & control
Echinococcus granulosus - genetics
Echinococcus granulosus - immunology
Female
Flow Cytometry
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Humans
Immunization
Immunoglobulin G - blood
Invertebrates
Lymphocyte Activation
Mice
Mice, Inbred BALB C
T-Lymphocytes - immunology
Vaccines - administration & dosage
Vaccines - genetics
Vaccines - immunology
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Summary:Here, we investigate in mice the immunogenicity of two antigens EgA31 and EgTrp which are expressed by the larval stage of Echinococcus granulosus. These recombinant proteins were used alone or as a mixture (EgA31-EgTrp) to immunize BALB/c mice. By flow cytometry, we have shown that the ratio CD4+/CD8+ of splenocytes were significantly higher in the antigen-immunized groups. The specific antibody in the sera and cytokine producing splenocytes was evaluated by enzyme-linked immunosorbent assay. EgA31, EgTrp or EgA31-EgTrp elicited high antibody titer of IgG and IgA. Among IgG isotypes, IgG1 was predominant for each antigen tested alone or combined. The production of IL-12, IFN-gamma, IL-10 and IL-6 cytokines was significantly higher in mice immunized with recombinant proteins. Our results suggest that, in BALB/c mice, a mixed Th1/Th2, response to EgA31, EgTrp and EgA31-EgTrp is obtained. The use of both antigens separately or in combination as candidate vaccine proteins is discussed.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-1322-x