Cells seeded on MBG scaffold survive impaction grafting technique: Potential application of cell-seeded biomaterials for revision arthroplasty

The objective of this study was to investigate the potential application of cell‐seeded biomaterials for revision arthroplasty and the reconstruction of major joints using the impaction grafting technique. Using morselized cancellous bone graft as a porous scaffold, MG63 cells were seeded on the sca...

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Bibliographic Details
Published in:Journal of orthopaedic research 2006-03, Vol.24 (3), p.501-507
Main Authors: Mushipe, Moses T., Chen, Xi, Jennings, David, Li, Gang
Format: Article
Language:English
Subjects:
allograft bone substitute
Animals
Arthroplasty, Replacement, Hip - instrumentation
Arthroplasty, Replacement, Hip - methods
Biocompatible Materials
Bone Transplantation
Cattle
Cell Adhesion
Cell Line, Tumor
Cell Survival
DNA - analysis
impaction grafting
Microscopy, Electron, Scanning
Osteocytes - transplantation
Osteocytes - ultrastructure
revision arthroplasty
revision arthroplasty, impaction grafting
Stress, Mechanical
tissue engineering
Tissue Engineering - methods
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Summary:The objective of this study was to investigate the potential application of cell‐seeded biomaterials for revision arthroplasty and the reconstruction of major joints using the impaction grafting technique. Using morselized cancellous bone graft as a porous scaffold, MG63 cells were seeded on the scaffold and impacted into an acetabulum cup model using a mechanical device constructed from data obtained during impaction grafting by an orthopedic surgeon. Immediately after impaction, cells were trypsinized from the scaffold and processed for cell survival rates using the double‐stranded DNA PicoGreen® assay. Significant reductions in viable cells were observed between the fifth impact and both the first and second impacts ( p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.20044