In vitro effects of high glucose concentrations on membrane protein oxidation, G-actin and deformability of human erythrocytes

The object of this study was to examine the effect of elevated in vitro glucose concentrations on protein modification and functional changes in human erythrocytes. Groups were exposed to 5–45 mM glucose concentrations. The time effect of any changes was also evaluated. In erythrocyte ghosts, protei...

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Bibliographic Details
Published in:Cell biochemistry and function 2005-05, Vol.23 (3), p.163-168
Main Authors: Resmi, Halil, Akhunlar, Hülya, Temiz Artmann, Ayşegül, Güner, Gül
Format: Article
Language:English
Subjects:
Actins - metabolism
Cisplatin - pharmacology
Cross-Linking Reagents - pharmacology
deformability
Dose-Response Relationship, Drug
erythrocyte
Erythrocyte Deformability - drug effects
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
G-actin
glucose
Glucose - pharmacology
glycation
Glycosylation
Humans
In Vitro Techniques
Oxidation-Reduction - drug effects
protein oxidation
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Summary:The object of this study was to examine the effect of elevated in vitro glucose concentrations on protein modification and functional changes in human erythrocytes. Groups were exposed to 5–45 mM glucose concentrations. The time effect of any changes was also evaluated. In erythrocyte ghosts, protein glycation and oxidation were evaluated using spectrophotometric methods. G‐actin was measured by a DNase I inhibition assay in cell lysates. Erythrocyte deformability was assessed using a cell transit analyser. At 24 h, a significant protein oxidation (at 25 and 45 mM glucose; p 
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.1129