Inhibitory Effects of the Flavonoids Isolated from Waltheria indica on the Production of NO, TNF-α and IL-12 in Activated Macrophages

Three flavonoids were isolated from the whole plants of Waltheria indica and biological properties investigated. On the basis of their spectroscopic data, these compounds were identified as (−)-epicatechin, quercetin, and tiliroside. These flavonoids significantly and dose-dependently inhibited the...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2005, Vol.28(5), pp.912-915
Main Authors: Rao, Yerra Koteswara, Fang, Shih-Hua, Tzeng, Yew-Min
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Dose-Response Relationship, Drug
Female
flavonoid
Flavonoids - chemistry
Flavonoids - isolation & purification
Flavonoids - pharmacology
interleukin-12
Interleukin-2 - antagonists & inhibitors
Interleukin-2 - biosynthesis
Macrophage Activation - drug effects
Macrophage Activation - physiology
Macrophages - drug effects
Macrophages - metabolism
Malvaceae
Mice
Mice, Inbred BALB C
nitric oxide
Nitric Oxide - antagonists & inhibitors
Nitric Oxide - biosynthesis
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - biosynthesis
tumor necrosis factor-α
Waltheria indica
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Summary:Three flavonoids were isolated from the whole plants of Waltheria indica and biological properties investigated. On the basis of their spectroscopic data, these compounds were identified as (−)-epicatechin, quercetin, and tiliroside. These flavonoids significantly and dose-dependently inhibited the production of the inflammatory mediator nitric oxide (NO), and the cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-12), in lipopolysaccharide (LPS) and interferon (IFN)-γ activated murine peritoneal macrophages, without displaying cytotoxicity. The order of inhibitory activity was quercetin>tiliroside>(−)-epicatechin. Furthermore, peritoneal macrophages were pre-activated with LPS/IFN-γ for 24 h, and the inhibitory effects of the above mentioned isolates on the production of NO were determined after a further 24 h, to address the possible mechanisms of their action. The present study supports the use of W. indica for the treatment of inflammatory diseases in traditional medicine.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.28.912