Recombinant glutathione- S-transferase a major allergen from Alternaria alternata for clinical use in allergy patients

Recombinant proteins are used for vaccines, therapy and diagnosis of many diseases. Biological activity of these may differ from native counterpart and needs investigation. The present study aimed to compare recombinant (r) and native (n) glutathione- S-transferase (GST) from Alternaria alternata. G...

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Bibliographic Details
Published in:Molecular Immunology 2006-05, Vol.43 (12), p.1927-1932
Main Authors: Shankar, Jay, Singh, Bhanu P., Gaur, Shailendra N., Arora, Naveen
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allergens - chemistry
Allergens - genetics
Allergens - immunology
Allergy
Alternaria - enzymology
Alternaria - genetics
Alternaria - immunology
Alternaria alternata
Case-Control Studies
Diagnosis
Female
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - immunology
Fungus
Glutathione Transferase - genetics
Glutathione Transferase - immunology
Glutathione Transferase - isolation & purification
Humans
Intradermal Tests
Male
Middle Aged
Recombinant allergens
Recombinant Proteins - immunology
Recombinant Proteins - isolation & purification
Rhinitis, Allergic, Seasonal - immunology
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Summary:Recombinant proteins are used for vaccines, therapy and diagnosis of many diseases. Biological activity of these may differ from native counterpart and needs investigation. The present study aimed to compare recombinant (r) and native (n) glutathione- S-transferase (GST) from Alternaria alternata. Glutathione- S-transferase sequence showed an ORF of 696 bp encoding 26-kDa protein with N-terminus conserved domain. Secondary structure of both forms was comparable with melting temperature of 57 and 59 °C, respectively. rGST and nGST showed similar enzymatic activity, allergenicity and potency by ELISA inhibition. Histamine release was comparable in 14/17 patients for both the GSTs. rGST and nGST induced proliferation in PBMC at different concentration. Cell supernatant revealed higher IL-4 and IL-5 levels with low levels of IFN-γ. In summary, recombinant and native GST demonstrated similar physio-chemical, biological and immunological properties and induced comparable cell mediated and humoral response to be used for diagnosis and specific immunotherapy for the fungal allergy cases.
ISSN:0161-5890
1872-9142
1365-2567
DOI:10.1016/j.molimm.2005.12.006