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Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)
Objectives: To determine the β-lactamase gene content of β-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment. Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic...
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| Published in: | Journal of antimicrobial chemotherapy 2005-05, Vol.55 (5), p.680-685 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | Poirel, Laurent Héritier, Claire Nordmann, Patrice |
| description | Objectives: To determine the β-lactamase gene content of β-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment. Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes. Results: Metallo-β-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B β-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum β-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid. Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of β-lactam resistance genes. |
| doi_str_mv | 10.1093/jac/dki065 |
| format | article |
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Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes. Results: Metallo-β-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B β-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum β-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid. Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of β-lactam resistance genes.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dki065</identifier><identifier>PMID: 15772146</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; beta-Lactam Resistance ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; beta-Lactams - pharmacology ; Biological and medical sciences ; carbapenems ; Chromosomes, Bacterial - genetics ; Cloning, Molecular ; Escherichia coli ; Medical sciences ; metallo-β-lactamases ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Pharmacology. Drug treatments ; Pseudomonas aeruginosa ; S. frigidimarina ; S. livingstonensis ; Sequence Analysis, DNA ; Shewanella - classification ; Shewanella - drug effects ; Shewanella - enzymology ; Shewanella - genetics ; Shewanella frigidimarina ; Shewanella livingstonensis</subject><ispartof>Journal of antimicrobial chemotherapy, 2005-05, Vol.55 (5), p.680-685</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-e159cadb8f574b8e97b2825c0e59dc2b63d852d472a3e746a2305e035fa8d9413</citedby><cites>FETCH-LOGICAL-c384t-e159cadb8f574b8e97b2825c0e59dc2b63d852d472a3e746a2305e035fa8d9413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16779100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15772146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poirel, Laurent</creatorcontrib><creatorcontrib>Héritier, Claire</creatorcontrib><creatorcontrib>Nordmann, Patrice</creatorcontrib><title>Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J. Antimicrob. Chemother</addtitle><description>Objectives: To determine the β-lactamase gene content of β-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment. Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes. Results: Metallo-β-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B β-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum β-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid. Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of β-lactam resistance genes.</description><subject>Amino Acid Sequence</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>beta-Lactam Resistance</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>beta-Lactams - pharmacology</subject><subject>Biological and medical sciences</subject><subject>carbapenems</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Cloning, Molecular</subject><subject>Escherichia coli</subject><subject>Medical sciences</subject><subject>metallo-β-lactamases</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Pharmacology. Drug treatments</subject><subject>Pseudomonas aeruginosa</subject><subject>S. frigidimarina</subject><subject>S. livingstonensis</subject><subject>Sequence Analysis, DNA</subject><subject>Shewanella - classification</subject><subject>Shewanella - drug effects</subject><subject>Shewanella - enzymology</subject><subject>Shewanella - genetics</subject><subject>Shewanella frigidimarina</subject><subject>Shewanella livingstonensis</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqF0c1u1DAQB_AIgehSuPAAyBcQIIX6M06ObEVbpJV6aEGISzSxJ123id3aWb7ehxfgQXgmnO6KcuNkyf55PON_UTxl9A2jjTi4BHNgrxyt1L1iwWRFS04bdr9YUEFVqaUSe8WjlC4pzaSqHxZ7TGnNs1wUP4_R4-QMAW9J54JZ4-gMDMSsIYKZMLofMLngSejJtMa8H8MYUhixRG-CRUvMACmRJfn9qxzyFRghYSJ9duRsjV_B4zAAGdwX5y_SFDz65BJ5ebZaluzV7cP_sD66C2fdCNF5yOhoRo-LBz0MCZ_s1v3iw9G788OTcnV6_P7w7ao0opZTiUw1BmxX90rLrsZGd7zmylBUjTW8q4StFbdScxCoZQU8fxBSoXqobSOZ2C9ebOtex3CzwTS1o0tm7stj2KS20jWtBa_-C5mWjaZqhq-30MSQUsS-vY7zcN9bRts5vTan127Ty_jZruqmG9He0V1cGTzfAUg5pD6CNy7duUrrhlGaXbl1Lk347e85xKs8gtCqPfn0ueWa1x-lPG-X4g-O1LQa</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Poirel, Laurent</creator><creator>Héritier, Claire</creator><creator>Nordmann, Patrice</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)</title><author>Poirel, Laurent ; Héritier, Claire ; Nordmann, Patrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-e159cadb8f574b8e97b2825c0e59dc2b63d852d472a3e746a2305e035fa8d9413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>beta-Lactam Resistance</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>beta-Lactams - pharmacology</topic><topic>Biological and medical sciences</topic><topic>carbapenems</topic><topic>Chromosomes, Bacterial - genetics</topic><topic>Cloning, Molecular</topic><topic>Escherichia coli</topic><topic>Medical sciences</topic><topic>metallo-β-lactamases</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Pharmacology. Drug treatments</topic><topic>Pseudomonas aeruginosa</topic><topic>S. frigidimarina</topic><topic>S. livingstonensis</topic><topic>Sequence Analysis, DNA</topic><topic>Shewanella - classification</topic><topic>Shewanella - drug effects</topic><topic>Shewanella - enzymology</topic><topic>Shewanella - genetics</topic><topic>Shewanella frigidimarina</topic><topic>Shewanella livingstonensis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poirel, Laurent</creatorcontrib><creatorcontrib>Héritier, Claire</creatorcontrib><creatorcontrib>Nordmann, Patrice</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poirel, Laurent</au><au>Héritier, Claire</au><au>Nordmann, Patrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J. Antimicrob. Chemother</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>55</volume><issue>5</issue><spage>680</spage><epage>685</epage><pages>680-685</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives: To determine the β-lactamase gene content of β-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment. Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes. Results: Metallo-β-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B β-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum β-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid. Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of β-lactam resistance genes.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>15772146</pmid><doi>10.1093/jac/dki065</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of antimicrobial chemotherapy, 2005-05, Vol.55 (5), p.680-685 |
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| language | eng |
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| source | Oxford Journals Online |
| subjects | Amino Acid Sequence Antibiotics. Antiinfectious agents. Antiparasitic agents beta-Lactam Resistance beta-Lactamases - genetics beta-Lactamases - metabolism beta-Lactams - pharmacology Biological and medical sciences carbapenems Chromosomes, Bacterial - genetics Cloning, Molecular Escherichia coli Medical sciences metallo-β-lactamases Microbial Sensitivity Tests Molecular Sequence Data Pharmacology. Drug treatments Pseudomonas aeruginosa S. frigidimarina S. livingstonensis Sequence Analysis, DNA Shewanella - classification Shewanella - drug effects Shewanella - enzymology Shewanella - genetics Shewanella frigidimarina Shewanella livingstonensis |
| title | Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1) |
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