Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation

Legionella pneumophila is a facultative intracellular human pathogen and an important cause of Legionnaires’ disease, a severe form of pneumonia. Recently, we showed the presence of a putative twin-arginine translocation (Tat) pathway in L. pneumophila Philadelphia-1. This secretion pathway is used...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-06, Vol.331 (4), p.1413-1420
Main Authors: De Buck, Emmy, Maes, Liesbeth, Meyen, Eef, Van Mellaert, Lieve, Geukens, Nick, Anné, Jozef, Lammertyn, Elke
Format: Article
Language:English
Subjects:
Acanthamoeba castellanii
Amino Acid Sequence
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Biofilms
Humans
Legionella pneumophila
Legionella pneumophila - enzymology
Legionella pneumophila - growth & development
Molecular Sequence Data
Signal peptides
Substrate Specificity
Tat secretion
Twin arginine
U937 Cells
Virulence
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Summary:Legionella pneumophila is a facultative intracellular human pathogen and an important cause of Legionnaires’ disease, a severe form of pneumonia. Recently, we showed the presence of a putative twin-arginine translocation (Tat) pathway in L. pneumophila Philadelphia-1. This secretion pathway is used to transport completely folded proteins across the cytoplasmic membrane. The importance of the Tat pathway in L. pneumophila was investigated by constructing a tatB and a tatC mutant. Functionality of the Tat pathway was shown using a proven heterologous Tat substrate. It was shown that tatB and tatC are involved in intracellular replication in Acanthamoeba castellanii and differentiated U937 cells, and in biofilm forming ability. A putative Legionella Tat substrate was identified via 2D gel electrophoresis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.04.060