Mouse genetic background influences severity of immune responses following trauma-hemorrhage

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2005-05, Vol.30 (4), p.168-176
• Main Authors: Matsutani, Takeshi, Anantha Samy, T.S., Kang, Shih-Ching, Bland, Kirby I., Chaudry, Irshad H.
• Format: Article
• Language: English
• Subjects: Animals; Bone Marrow Cells; Bone-marrow proliferation; Cell Proliferation; Cytokine release; Genetic Predisposition to Disease; Hemorrhage - genetics; Hemorrhage - immunology; Immunity, Cellular - genetics; Interferon-gamma - metabolism; Interleukin-10 - metabolism; Interleukin-2 - metabolism; Macrophages - metabolism; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Spleen - cytology; Spleen - immunology; Spleen - metabolism; Splenocytes; Tumor Necrosis Factor-alpha - metabolism; Wounds and Injuries - genetics; Wounds and Injuries - immunology
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2004.12.019

Studies have shown that following bacterial infection or endotoxin administration, immune functions are regulated differently in mice of different genetic background. Since the susceptibility to sepsis following trauma-hemorrhage is dependant on the severity of injury, it is important to determine whether genetic background of the animal influence immune functions after trauma-hemorrhage. The aim of our studies, therefore, was to assess differences in the immune functions in genetically different strains of age-matched C3H/HeN and C57BL/6 male mice following trauma-hemorrhage. The analysis for immune functions included: proliferation of splenocyte and bone-marrow cells, IL-2 and IFN-γ release by splenocytes, and TNF-α and IL-10 release by splenic, peritoneal, liver (Kupffer cell), and bone-marrow macrophages. The results show significant differences in splenocyte and bone-marrow functions, and in the release of the mediators of immune function by immune competent cells: (a) between the two genetic strains, and (b) in each mouse strain following trauma-hemorrhage. Thus, genetic background appears to significantly influence the severity of immune responses in males following trauma-hemorrhage.