Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes

Recombinant Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP) 3 as well as two T cell epitopes of the M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Imm...

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Bibliographic Details
Published in:Vaccine 2006-04, Vol.24 (17), p.3646-3653
Main Authors: Rapeah, S., Norazmi, M.N.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Bacterial - immunology
Applied microbiology
Bacterial diseases
Bacterial Proteins
BCG Vaccine - immunology
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - immunology
Cytokines - biosynthesis
Epitopes, T-Lymphocyte
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Human protozoal diseases
Immunization
Immunoglobulin G - blood
Immunoglobulin G - classification
Infectious diseases
Lymphocyte Activation
Malaria
Malaria Vaccines - immunology
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Molecular Sequence Data
Mycobacterium bovis
Mycobacterium tuberculosis
Oligopeptides - genetics
Oligopeptides - immunology
Parasitic diseases
Protozoal diseases
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Recombinant BCG
Tuberculosis
Tuberculosis and atypical mycobacterial infections
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Synthetic - immunology
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Description
Summary:Recombinant Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP) 3 as well as two T cell epitopes of the M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Immunised Balb/c mice demonstrated an increase in almost all of the IgG subclasses against both malarial epitopes and enhanced splenocyte proliferative response against the malarial epitopes as well as selected peptides of ESAT-6. Furthermore, flow cytometric analyses showed elevated numbers of CD4 + lymphocytes expressing IFN-γ and IL-2 against the ESAT-6 peptides, suggesting a specific Th1-mediated response. This study demonstrated that expressing malarial and TB epitopes in a single rBCG construct induced appropriate humoral and cellular immune response against immunogenic epitopes from both organisms.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2006.01.053