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Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes
Recombinant Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP) 3 as well as two T cell epitopes of the M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Imm...
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| Published in: | Vaccine 2006-04, Vol.24 (17), p.3646-3653 |
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| cited_by | cdi_FETCH-LOGICAL-c424t-52f8eb8ce2d1cf0c9a38e86d1fe4eeb52c35b5b81793f09ac7e4fb4dfb0b71b03 |
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| container_end_page | 3653 |
| container_issue | 17 |
| container_start_page | 3646 |
| container_title | Vaccine |
| container_volume | 24 |
| creator | Rapeah, S. Norazmi, M.N. |
| description | Recombinant
Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP)
3 as well as two T cell epitopes of the
M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Immunised Balb/c mice demonstrated an increase in almost all of the IgG subclasses against both malarial epitopes and enhanced splenocyte proliferative response against the malarial epitopes as well as selected peptides of ESAT-6. Furthermore, flow cytometric analyses showed elevated numbers of CD4
+ lymphocytes expressing IFN-γ and IL-2 against the ESAT-6 peptides, suggesting a specific Th1-mediated response. This study demonstrated that expressing malarial and TB epitopes in a single rBCG construct induced appropriate humoral and cellular immune response against immunogenic epitopes from both organisms. |
| doi_str_mv | 10.1016/j.vaccine.2006.01.053 |
| format | article |
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Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP)
3 as well as two T cell epitopes of the
M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Immunised Balb/c mice demonstrated an increase in almost all of the IgG subclasses against both malarial epitopes and enhanced splenocyte proliferative response against the malarial epitopes as well as selected peptides of ESAT-6. Furthermore, flow cytometric analyses showed elevated numbers of CD4
+ lymphocytes expressing IFN-γ and IL-2 against the ESAT-6 peptides, suggesting a specific Th1-mediated response. This study demonstrated that expressing malarial and TB epitopes in a single rBCG construct induced appropriate humoral and cellular immune response against immunogenic epitopes from both organisms.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2006.01.053</identifier><identifier>PMID: 16494975</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Antigens, Bacterial - immunology ; Applied microbiology ; Bacterial diseases ; Bacterial Proteins ; BCG Vaccine - immunology ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - immunology ; Cytokines - biosynthesis ; Epitopes, T-Lymphocyte ; Fundamental and applied biological sciences. Psychology ; Human bacterial diseases ; Human protozoal diseases ; Immunization ; Immunoglobulin G - blood ; Immunoglobulin G - classification ; Infectious diseases ; Lymphocyte Activation ; Malaria ; Malaria Vaccines - immunology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Molecular Sequence Data ; Mycobacterium bovis ; Mycobacterium tuberculosis ; Oligopeptides - genetics ; Oligopeptides - immunology ; Parasitic diseases ; Protozoal diseases ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Recombinant BCG ; Tuberculosis ; Tuberculosis and atypical mycobacterial infections ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Synthetic - immunology</subject><ispartof>Vaccine, 2006-04, Vol.24 (17), p.3646-3653</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-52f8eb8ce2d1cf0c9a38e86d1fe4eeb52c35b5b81793f09ac7e4fb4dfb0b71b03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17665916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16494975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rapeah, S.</creatorcontrib><creatorcontrib>Norazmi, M.N.</creatorcontrib><title>Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Recombinant
Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP)
3 as well as two T cell epitopes of the
M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Immunised Balb/c mice demonstrated an increase in almost all of the IgG subclasses against both malarial epitopes and enhanced splenocyte proliferative response against the malarial epitopes as well as selected peptides of ESAT-6. Furthermore, flow cytometric analyses showed elevated numbers of CD4
+ lymphocytes expressing IFN-γ and IL-2 against the ESAT-6 peptides, suggesting a specific Th1-mediated response. This study demonstrated that expressing malarial and TB epitopes in a single rBCG construct induced appropriate humoral and cellular immune response against immunogenic epitopes from both organisms.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins</subject><subject>BCG Vaccine - immunology</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - immunology</subject><subject>Cytokines - biosynthesis</subject><subject>Epitopes, T-Lymphocyte</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human bacterial diseases</subject><subject>Human protozoal diseases</subject><subject>Immunization</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - classification</subject><subject>Infectious diseases</subject><subject>Lymphocyte Activation</subject><subject>Malaria</subject><subject>Malaria Vaccines - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium tuberculosis</subject><subject>Oligopeptides - genetics</subject><subject>Oligopeptides - immunology</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Recombinant BCG</subject><subject>Tuberculosis</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkcGKFDEQhoMo7rj6CEoueus26U6nu08ig7surHhR8BaSdGXJkE7GJD3sgAffYV_C9_BNfBKzTMMe91QUfH9VUR9CrympKaH8_a4-SK2th7ohhNeE1qRrn6ANHfq2ajo6PEUb0nBWMUp-nKEXKe0IKQgdn6MzytnIxr7boF9X87z4cAPeapuPOBgscQQdZmW99Bl_OeqgpM4Q7TJjFQ424dJb5wBvpZshZ_j3--5y-fsnWo_hdh8hJetv8CydjFY6LP2E86Ig6sWFVPKwtznsIb1Ez4x0CV6t9Rx9v_j0bfu5uv56ebX9eF1p1rBcdY0ZQA0amolqQ_Qo2wEGPlEDDEB1jW471amB9mNryCh1D8woNhlFVE8Vac_Ru9PcfQw_F0hZzDZpcE56CEsSvB8oJbx5FKQ9LS9krIDdCdQxpBTBiH20s4xHQYm49yN2YvUj7v0IQkVJltybdcGiZpgeUquQArxdAZm0dCZKr2164HrOu5Hywn04cVD-drAQRdIWvIbJFntZTME-csp_Ey-2xA</recordid><startdate>20060424</startdate><enddate>20060424</enddate><creator>Rapeah, S.</creator><creator>Norazmi, M.N.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060424</creationdate><title>Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes</title><author>Rapeah, S. ; Norazmi, M.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-52f8eb8ce2d1cf0c9a38e86d1fe4eeb52c35b5b81793f09ac7e4fb4dfb0b71b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Bacterial diseases</topic><topic>Bacterial Proteins</topic><topic>BCG Vaccine - immunology</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - immunology</topic><topic>Cytokines - biosynthesis</topic><topic>Epitopes, T-Lymphocyte</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human bacterial diseases</topic><topic>Human protozoal diseases</topic><topic>Immunization</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - classification</topic><topic>Infectious diseases</topic><topic>Lymphocyte Activation</topic><topic>Malaria</topic><topic>Malaria Vaccines - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium tuberculosis</topic><topic>Oligopeptides - genetics</topic><topic>Oligopeptides - immunology</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Recombinant BCG</topic><topic>Tuberculosis</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rapeah, S.</creatorcontrib><creatorcontrib>Norazmi, M.N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rapeah, S.</au><au>Norazmi, M.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-04-24</date><risdate>2006</risdate><volume>24</volume><issue>17</issue><spage>3646</spage><epage>3653</epage><pages>3646-3653</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Recombinant
Mycobacterium bovis bacille Calmette–Guèrin (rBCG) expressing the malarial epitopes F2R(II)EBA and (NANP)
3 as well as two T cell epitopes of the
M. tuberculosis ESAT-6 antigen, generated in favour of mycobacterium codon usage elicited specific immune response against these epitopes. Immunised Balb/c mice demonstrated an increase in almost all of the IgG subclasses against both malarial epitopes and enhanced splenocyte proliferative response against the malarial epitopes as well as selected peptides of ESAT-6. Furthermore, flow cytometric analyses showed elevated numbers of CD4
+ lymphocytes expressing IFN-γ and IL-2 against the ESAT-6 peptides, suggesting a specific Th1-mediated response. This study demonstrated that expressing malarial and TB epitopes in a single rBCG construct induced appropriate humoral and cellular immune response against immunogenic epitopes from both organisms.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16494975</pmid><doi>10.1016/j.vaccine.2006.01.053</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Antigens, Bacterial - immunology Applied microbiology Bacterial diseases Bacterial Proteins BCG Vaccine - immunology Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - immunology Cytokines - biosynthesis Epitopes, T-Lymphocyte Fundamental and applied biological sciences. Psychology Human bacterial diseases Human protozoal diseases Immunization Immunoglobulin G - blood Immunoglobulin G - classification Infectious diseases Lymphocyte Activation Malaria Malaria Vaccines - immunology Medical sciences Mice Mice, Inbred BALB C Microbiology Molecular Sequence Data Mycobacterium bovis Mycobacterium tuberculosis Oligopeptides - genetics Oligopeptides - immunology Parasitic diseases Protozoal diseases Protozoan Proteins - genetics Protozoan Proteins - immunology Recombinant BCG Tuberculosis Tuberculosis and atypical mycobacterial infections Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Synthetic - immunology |
| title | Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette–Guèrin expressing malarial and tuberculosis epitopes |
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