Reflective Interferometric Fourier Transform Spectroscopy:  A Self-Compensating Label-Free Immunosensor Using Double-Layers of Porous SiO2

An interferometric biosensor comprised of two layers of porous Si, stacked one on top of the other, is described. A fast Fourier transform (FFT) of the reflectivity spectrum reveals three peaks that correspond to the optical thickness of the top layer, the bottom layer, and both layers together. Bin...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-04, Vol.128 (13), p.4250-4252
Main Authors: Pacholski, Claudia, Yu, Christine, Miskelly, Gordon M, Godin, Denis, Sailor, Michael J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Fourier Analysis
Fundamental and applied biological sciences. Psychology
Immunoglobulin G - chemistry
Interferometry - methods
Methods. Procedures. Technologies
Rabbits
Silicon Dioxide - chemistry
Staphylococcal Protein A - analysis
Various methods and equipments
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Summary:An interferometric biosensor comprised of two layers of porous Si, stacked one on top of the other, is described. A fast Fourier transform (FFT) of the reflectivity spectrum reveals three peaks that correspond to the optical thickness of the top layer, the bottom layer, and both layers together. Binding of immunoglobulin G to a protein A capture probe adsorbed to the surface of the top layer induces changes in reflectivity at the top layer/solution interface. The FFT method allows discrimination of target analyte binding from matrix effects due to nonspecific changes in the analyte solution. The sensor response is shown to be insensitive to the addition of 4000-fold excess sucrose or 80-fold excess bovine serum albumin interferents.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja056702b