Microdevice DNA forensics by the simple tandem repeat method

We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Ful...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-04, Vol.1111 (2), p.206-213
Main Authors: Goedecke, Nils, McKenna, Brian, El-Difrawy, Sameh, Gismondi, Elizabeth, Swenson, Abigail, Carey, Loucinda, Matsudaira, Paul, Ehrlich, Daniel J.
Format: Article
Language:English
Subjects:
DNA - genetics
Forensic DNA analysis
Forensic Medicine
Humans
Microdevice electrophoresis
Microfluidics
Short tandem repeat
Tandem Repeat Sequences
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Summary:We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.05.100