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Vascular tenascin-C regulates cardiac endothelial phenotype and neovascularization
ABSTRACT Microenvironmental cues mediate postnatal neovascularization via modulation of endothelial cell and bone marrow‐derived endothelial progenitor cell (EPC) activity. Numerous signals regulate the activity of both of these cell types in response to vascular injury, which suggests that parallel...
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Published in: | The FASEB journal 2006-04, Vol.20 (6), p.717-719 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ABSTRACT
Microenvironmental cues mediate postnatal neovascularization via modulation of endothelial cell and bone marrow‐derived endothelial progenitor cell (EPC) activity. Numerous signals regulate the activity of both of these cell types in response to vascular injury, which suggests that parallel mechanisms regulate angiogenesis in the vascular beds of both the heart and bone marrow. To identify mediators of such shared pathways, in vivo bone marrow/cardiac phage display biopanning was performed and led to the identification of tenascin‐C as a candidate protein. Functionally, tenascin‐C inhibits cardiac endothelial cell spreading and enhances migration in response to angiogenic growth factors. Analysis of human coronary thrombi revealed tenascin‐C protein expression colocalized with the endothelial cell/EPC marker Tie‐2 in intrathrombi vascular channels. Immunostains in the rodent heart demonstrated that tenascin‐C also colocalizes with EPCs homing to sites of cardiac angiogenic induction. To determine the importance of tenascin‐C in cardiac neovascularization, we used an established cardiac transplantation model and showed that unlike wild‐type mice, tenascin‐C−/− mice fail to vascularize cardiac allografts. This demonstrates for the first time that tenascin‐C is essential for postnatal cardiac angiogenic function. Together, our data highlight the role of tenascin‐C as a microenvironmental regulator of cardiac endothelial/EPC activity. |
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ISSN: | 1530-6860 0892-6638 1530-6860 |
DOI: | 10.1096/fj.05-5131fje |