Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model

Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change...

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Published in:Acta cirúrgica brasileira 2006-04, Vol.21 (2), p.74-79
Main Authors: Araújo Filho, Irami, Honorato Sobrinho, Abraão Allen, Rego, Amália Cinthia Meneses do, Garcia, Ana Claudia M de Amorim, Fernandes, Daniele Pimentel, Cruz, Thaís Medeiros, Costa, Ticiana Cabral da, Medeiros, Aldo Cunha
Format: Article
Language:English
Subjects:
Animals
Carbon Dioxide - metabolism
Cytokines - metabolism
Laparoscopy - adverse effects
Laparotomy
Leukocyte Count
Leukocytes - metabolism
Male
Peritonitis - metabolism
Peritonitis - pathology
Peritonitis - surgery
Pneumoperitoneum, Artificial - adverse effects
Random Allocation
Rats
Rats, Wistar
Stress, Physiological - metabolism
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Summary:Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFalpha, IL-1beta, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exudates. Peritoneal TNFalpha, IL-1beta and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p
ISSN:0102-8650
0102-8650
DOI:10.1590/s0102-86502006000200004