Characterization of a Molt-Inhibiting Hormone (MIH) Receptor in the Y-Organ of the Kuruma Prawn, Marsupenaeus japonicus

: To characterize a receptor for molt‐inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross‐linking experiments using radiolabeled recombinant MIH ([125I]rMIH). The specific binding of [125I]rMIH was found in the membrane fraction of th...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2005-04, Vol.1040 (1), p.215-218
Main Authors: ASAZUMA, HIDEAKI, NAGATA, SHINJI, KATAYAMA, HIDEKAZU, OHIRA, TSUYOSHI, NAGASAWA, HIROMICHI
Format: Article
Language:English
Subjects:
Animals
Binding
Crosslinking
Endocrine Glands - metabolism
hormone receptor
Hormones
Invertebrate Hormones - chemistry
Invertebrate Hormones - isolation & purification
Invertebrate Hormones - metabolism
Marine
Marsupenaeus japonicus
Membranes
molt-inhibiting hormone
Neuropeptides - chemistry
Neuropeptides - isolation & purification
Neuropeptides - metabolism
Penaeidae - metabolism
Prawns
Protein Binding - physiology
Proteins
Receptors
Recombinant
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: To characterize a receptor for molt‐inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross‐linking experiments using radiolabeled recombinant MIH ([125I]rMIH). The specific binding of [125I]rMIH was found in the membrane fraction of the Y‐organ (Kd= 4.76 × 10−10 M and Bmax= 5.51 × 10−12 M). Chemical cross‐linking between [125I]rMIH and the membrane fraction of the Y‐organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1327.027