BKV Infection and Hemorrhagic Cystitis after Allogeneic Bone Marrow Transplant

Hemorrhagic cystitis (HC) is a well-known complication after allogeneic bone marrow transplant (BMT) and can be related to adenovirus or human polyomavirus BK (BKV) infections. In this study a group of 20 patients after allogeneic BMT has been examined. BMT urine samples were analysed for the presen...

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Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2005-04, Vol.18 (2), p.309-316
Main Authors: Fioriti, D., Degener, A.M., Mischitelli, M., Videtta, M., Arancio, A., Sica, S., Sorà, F., Pietropaolo, V.
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenoviridae - isolation & purification
Adenoviridae Infections - urine
Adenoviridae Infections - virology
Adult
Base Sequence
BK Virus - genetics
BK Virus - isolation & purification
Bone Marrow Transplantation
Cystitis - urine
Cystitis - virology
DNA, Viral - analysis
DNA, Viral - urine
Female
Hemorrhage - urine
Hemorrhage - virology
Humans
Locus Control Region - genetics
Male
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polyomavirus Infections - urine
Polyomavirus Infections - virology
Sequence Alignment
Transplantation, Homologous
Urine - virology
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Summary:Hemorrhagic cystitis (HC) is a well-known complication after allogeneic bone marrow transplant (BMT) and can be related to adenovirus or human polyomavirus BK (BKV) infections. In this study a group of 20 patients after allogeneic BMT has been examined. BMT urine samples were analysed for the presence of Adenovirus and BKV DNA by means of polymerase chain reaction (PCR). 5/20 BMT patients developed HC after BMT. The presence of BKV DNA in urine samples was evident in 3/15 patients without HC and in 5/5 patients with HC. In 2/5 HC-patients the BKV DNA was not found after therapy with Cidofovir and Ribavirin. The search for adenovirus DNA in all samples was negative. The analysis of BKV non-coding control region (NCCR) isolated from urine samples revealed a structure very similar to the archetype in all samples. The RFLP (Restriction Fragment Length Polymorphism assay) showed the presence of BKV subtypes I and IV, with the prevalence of subtype I (4/5). This study supports the hypothesis that HC is mainly related to BKV rather than to adenovirus infection in BMT patients. Moreover, since BKV subtype I was predominant, it is reasonable to hypothesize that a specific BKV subtype could be associated with the development of HC.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463200501800213