Fine mapping of a QTL region with large effects on growth and fatness on mouse chromosome 2

1 Department of Animal Science, University of Nebraska, Lincoln, Nebraska 2 North Carolina State University, Raleigh, North Carolina We combined the use of a congenic line and recombinant progeny testing (RPT) to characterize and fine map a previously identified region of distal mouse chromosome 2 (...

Full description

Saved in:
Bibliographic Details
Published in:Physiological genomics 2005-05, Vol.21 (3), p.411-422
Main Authors: Jerez-Timaure, Nancy C, Eisen, Eugene J, Pomp, Daniel
Format: Article
Language:English
Subjects:
Animals
Body Composition - genetics
Body Weight - genetics
Chromosome Mapping
Crosses, Genetic
Female
Genotype
Growth - genetics
Male
Mice
Obesity - genetics
Phenotype
Quantitative Trait Loci
Recombination, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1 Department of Animal Science, University of Nebraska, Lincoln, Nebraska 2 North Carolina State University, Raleigh, North Carolina We combined the use of a congenic line and recombinant progeny testing (RPT) to characterize and fine map a previously identified region of distal mouse chromosome 2 (MMU2) harboring quantitative trait loci (QTL) with large effects on growth and fatness. The congenic line [M16i.B6-( D2Mit306 - D2Mit52 ); MB2] was created using an inbred line (M16i) derived from a line that had undergone long-term selection for rapid weight gain (M16) as the recipient for an 38-cM region on MMU2 from the inbred line C57BL/6J. A large F 2 cohort (1,200 mice) originating from a cross between MB2 and M16i was created, and 40 F 2 males with defined recombinations within the QTL region were used to produce 665 segregating progeny. Linkage analysis of the F 2 population detected QTL with very large effects on body weight, body fat, lean tissue mass, bone mineral density, and liver weight. Confidence intervals of the QTL were narrowed to regions of 1.5–4.5 cM. Analysis of progeny of the recombinant F 2 males confirmed the existence of the QTL and further contributed to localization of their map positions. These efforts confirmed the presence of QTL with major effect on MMU2, narrowed the estimated region harboring the QTL from 38 to 12 cM, and further characterized phenotypic effects of the QTL, effectively culminating in a significantly decreased pool of positional candidate genes potentially representing these genes controlling predisposition to growth and fatness. fine mapping; quantitative trait locus; obesity; congenic; recombinant progeny testing
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.00256.2004