Study of applicability of the bifunctional system "ethidium bromide + Hoechst-33258" for DNA analysis

Changes in absorbance and fluorescence excitation and emission spectra in the ultraviolet and visible regions of the system containing ethidium bromide (EtBr) and Hoechst-33258 (Ht) were investigated depending on various DNA quantities and the composition of the medium. It is noted that spectral pro...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2005-04, Vol.70 (4), p.449-457
Main Author: Sibirtsev, V S
Format: Article
Language:English
Subjects:
Aging - genetics
Animals
Benzimidazoles - chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA, Superhelical - chemistry
Dyes
Energy transfer
Energy transformation
Ethidium - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Nucleic acids
Rats
Spectral energy distribution
Spectrum Analysis
Telomere - chemistry
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Summary:Changes in absorbance and fluorescence excitation and emission spectra in the ultraviolet and visible regions of the system containing ethidium bromide (EtBr) and Hoechst-33258 (Ht) were investigated depending on various DNA quantities and the composition of the medium. It is noted that spectral properties of this system are determined by interactions of EtBr and Ht both with nucleic acid and with one another (for example, joint sorption of EtBr and Ht on DNA may involve fluorescent resonance energy transfer between the dye molecules). Thus, different modes of EtBr and Ht specificity to substrate and assay conditions suggest that combined use of these dyes provides some additional effects that may be interesting in terms of structure-functional study of nucleic acid. Some of these effects are considered in this paper.
ISSN:0006-2979
1608-3040
0320-9725
DOI:10.1007/s10541-005-0136-x