Expression of ubiquitin-related enzymes in the suprachiasmatic nucleus with special reference to ubiquitin carboxy-terminal hydrolase UchL1

There is growing evidence that ubiquitin-proteasome system plays an important role for the generation of circadian rhythms in mice as in Drosophila. Here we examined the expression of ubiquitin-related enzymes (Ubce5, UbcM4, Ube2v, Ube2d2, UchL1, UchL3, Ubp41, Ufd1L, β-TrCP) in the suprachiasmatic n...

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Bibliographic Details
Published in:Biomedical Research 2005, Vol.26(2), pp.43-49
Main Authors: DONG, Xin, YAGITA, Kazuhiro, ZHANG, Jing, OKAMURA, Hitoshi
Format: Article
Language:English
Subjects:
Animals
Cell Line
Circadian Rhythm
Darkness
DNA Primers - chemistry
DNA, Complementary - metabolism
Drosophila
Fibroblasts - metabolism
Hydrolases - biosynthesis
In Situ Hybridization
Light
Male
Mice
Mice, Inbred C57BL
Nucleic Acid Hybridization
Proteasome Endopeptidase Complex - metabolism
RNA - metabolism
RNA, Messenger - metabolism
Suprachiasmatic Nucleus - metabolism
Time Factors
Ubiquitin - chemistry
Ubiquitin - metabolism
Ubiquitin Thiolesterase - biosynthesis
Ubiquitin Thiolesterase - chemistry
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Summary:There is growing evidence that ubiquitin-proteasome system plays an important role for the generation of circadian rhythms in mice as in Drosophila. Here we examined the expression of ubiquitin-related enzymes (Ubce5, UbcM4, Ube2v, Ube2d2, UchL1, UchL3, Ubp41, Ufd1L, β-TrCP) in the suprachiasmatic nucleus (SCN). At mRNA level, the expression of these enzymes were faint to moderate except ubiquitin carboxy-terminal hydrolase L1 (UchL1), a dominant deubiquitinating salvaging enzyme. Although strongly expressed in the SCN, UchL1 mRNA did not show the rhythm in the SCN in both light-dark and constant dark conditions.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.26.43