Differentiation of Borrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis

Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorf...

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Bibliographic Details
Published in:Electrophoresis 2006-04, Vol.27 (7), p.1322-1330
Main Authors: Simenc, Janez, Ruzić-Sabljić, Eva, Avgustin, Gorazd
Format: Article
Language:English
Subjects:
Borrelia burgdorferi Group - classification
Borrelia burgdorferi Group - genetics
Borrelia burgdorferi Group - isolation & purification
Borrelia burgdorferi sensu lato
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Electrophoresis, Polyacrylamide Gel - methods
PCR
PFGE
Species differentiation
Temperature
TGGE
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Summary:Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S‐23S intergenic space region PCR product. A differentiation of B. garinii, B. afzelii, and B. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S‐23S intergenic space region PCR product showed no difference in specificity of species identification.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200500373