Expression of the human FUSED protein in Drosophila

The Drosophila segment polarity gene fused, which encodes a serine threonine kinase, is required to transmit the Hedgehog (Hh) signal in imaginal discs. To explore the functional homology between the human protein FUSED (hFU) and the Drosophila protein fused (dFu), we have subjected hFU to a precise...

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Bibliographic Details
Published in:Development genes and evolution 2005-05, Vol.215 (5), p.230-237
Main Authors: Daoud, Fatma, Blanchet-Tournier, Marie-Françoise
Format: Article
Language:English
Subjects:
Animals
Body Patterning
Cells, Cultured
Drosophila
Drosophila - genetics
Drosophila - metabolism
Gene Expression Regulation, Developmental
Humans
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
Kinases
Mice
Mutation
NIH 3T3 Cells
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Recombinant Proteins - metabolism
Sequence Alignment
Signal Transduction
Transfection
Transgenes
Wings, Animal - metabolism
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Summary:The Drosophila segment polarity gene fused, which encodes a serine threonine kinase, is required to transmit the Hedgehog (Hh) signal in imaginal discs. To explore the functional homology between the human protein FUSED (hFU) and the Drosophila protein fused (dFu), we have subjected hFU to a precise and well-defined Hh signalling assay of Drosophila wing development. In the wildtype, hFU affects the expression of Hh target genes leading thus to defects in adult wings. In fu mutants, overexpression of hFU cannot rescue the fu phenotype. These results suggest that hFU in Drosophila interferes with endogenous Hh signalling probably by competing with endogenous dFu when binding its partners but cannot perform the normal Fu function.
ISSN:0949-944X
1432-041X
DOI:10.1007/s00427-005-0469-x