Molecular cloning and characterization of a novel MAP kinase gene in  Chorispora bungeana

• Published in: Plant physiology and biochemistry 2006, Vol.44 (1), p.78-84
• Main Authors: Zhang, Tengguo, Liu, Yubing, Xue, Lingui, Xu, Shijian, Chen, Tuo, Yang, Tongwen, Zhang, Lijing, An, Lizhe
• Format: Article
• Language: English
• Subjects: Abscisic Acid - chemistry; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Brassicaceae - enzymology; Chorispora; Chorispora bungeana; Cloning, Molecular; Cold Temperature; Environmental stresses; Enzymes; Fundamental and applied biological sciences. Psychology; Metabolism; Mitogen-activated protein kinases; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Molecular Sequence Data; Phosphorylation; Phylogeny; Plant Leaves - enzymology; Plant physiology and development; Plant Roots - enzymology; RACE; RNA, Messenger - metabolism; Salts - pharmacology; Sequence Homology, Amino Acid
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2006.01.001

Chorispora bungeana Fisch. and C.A. Mey ( Chorispora bungeana) is a rare alpine subnival plant species that is highly capable of resisting freezing environment. Since it is a stress-tolerant plant, we investigated the participation of mitogen-activated protein kinases (MAPKs) as possible mediators of abiotic stresses. We have isolated from Chorispora bungeana a new MAPK cDNA CbMAPK3 which encodes a 369 amino-acid protein with moderate to high nucleotide sequence similarity to previously reported plant MAPK genes. CbMAPK3 contains all 11 of the MAPK conserved subdomains and the phosphorylation motif TEY. The transcripts of CbMAPK3 were detected and no tissue-specific expression were observed in both roots and leaves, The transcripts of CbMAPK3 accumulated highly and rapidly when Chorispora bungeana treated with cold (4 and –4 °C), ABA and salinity stress. These results indicate that the CbMAPK3 may play an important role in response to environmental stresses.