Oxidase reaction of the hybrid Mn-peroxidase of the fungus Panus tigrinus 8/18

The hybrid Mn-peroxidase of the fungus Panus tigrinus 8/18 oxidized NADH in the absence of hydrogen peroxide, this being accompanied by the consumption of oxygen. The reaction of NADH oxidation started after a period of induction and completely depended on the presence of Mn(II). The reaction was in...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2005-04, Vol.70 (4), p.467-472
Main Authors: Lisov, A V, Leontievsky, A A, Golovleva, L A
Format: Article
Language:English
Subjects:
Absorption spectra
Analysis
Bacteria
Bleaching
Catalase - metabolism
Catalysis
Enzyme Activation
Hydrogen
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Hydrogen production
Inactivation
Lentinula - enzymology
Lentinula - metabolism
Manganese
Manganese - metabolism
NAD - metabolism
Oxidation
Oxidation-Reduction
Oxygen Consumption
Peroxidases - metabolism
Spectra
Superoxide Dismutase - metabolism
Superoxides - metabolism
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Summary:The hybrid Mn-peroxidase of the fungus Panus tigrinus 8/18 oxidized NADH in the absence of hydrogen peroxide, this being accompanied by the consumption of oxygen. The reaction of NADH oxidation started after a period of induction and completely depended on the presence of Mn(II). The reaction was inhibited in the presence of catalase and superoxide dismutase. Oxidation of NADH by the enzyme or by manganese(III)acetate was accompanied by the production of hydrogen peroxide and superoxide radicals. In the presence of NADH, the enzyme was transformed into a catalytically inactive oxidized form (compound III), and the latter was inactivated with bleaching of the heme. The substrate of the hybrid Mn-peroxidase (Mn(II)) reduced compound III to yield the native form of the enzyme and prevented its inactivation. It is assumed that the hybrid Mn-peroxidase used the formed hydrogen peroxide in the usual peroxidase reaction to produce Mn(III), which was involved in the formation of hydrogen peroxide and thus accelerated the peroxidase reaction. The reaction of NADH oxidation is a peroxidase reaction and the consumption of oxygen is due to its interaction with the products of NADH oxidation. The role of Mn(II) in the oxidation of NADH consisted in the production of hydrogen peroxide and the protection of the enzyme from inactivation.
ISSN:0006-2979
1608-3040
0320-9725
DOI:10.1007/s10541-005-0138-8