Identification and characterization of collagen-binding activity in Streptococcus mutans wall-associated protein: A possible implication in dental root caries and endocarditis

Streptococcus mutans is implicated in coronal and dental root decay, and in endocarditis. Comparative study of the amino acid sequence of S. mutans 47 kDa wall-associated protein A (WapA) revealed a collagen-binding domain (CBD) at the N-terminal region. Recombinant AgA (WapA truncated at the carbox...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-05, Vol.343 (3), p.787-792
Main Authors: Han, Thomas K., Zhang, Chi, Dao, My Lien
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibodies, Bacterial - pharmacology
Antigens, Bacterial - chemistry
Antigens, Bacterial - immunology
Antigens, Bacterial - metabolism
Bacterial Proteins - chemistry
Bacterial Proteins - immunology
Bacterial Proteins - metabolism
Binding Sites
Collagen Type I - metabolism
Collagen-binding activity
Consensus Sequence
Dental root decay
Endocarditis
Endocarditis, Bacterial - microbiology
Molecular Sequence Data
Protein Structure, Tertiary
Root Caries - microbiology
Sequence Alignment
Streptococcus mutans
Streptococcus mutans - immunology
Streptococcus mutans - pathogenicity
Wall-associated protein A
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Summary:Streptococcus mutans is implicated in coronal and dental root decay, and in endocarditis. Comparative study of the amino acid sequence of S. mutans 47 kDa wall-associated protein A (WapA) revealed a collagen-binding domain (CBD) at the N-terminal region. Recombinant AgA (WapA truncated at the carboxyterminal end) was isolated, biotin-labeled, and analyzed by Solid Phase Binding Assay. The results showed that biotin-labeled AgA bound significantly and in a dose-dependent manner to immobilized collagen type I, and to a lesser extent to fibronectin, but not to collagen type IV or laminin. Binding of biotin-labeled S. mutans cells to collagen-coated surfaces was significantly inhibited by antibody to WapA or AgA ( P < 0.001). The results obtained confirmed the collagen-binding activity of CBD in AgA and WapA, and suggested that WapA may be used, not only as a vaccine against coronal and dental root caries, but also against S. mutans-mediated endocarditis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.03.025