Measurement of free thyroxine concentration in horses by equilibrium dialysis

The purpose of the study reported here was to validate measurement of free thyroxine (fT4) concentration in equine serum by equilibrium dialysis (fT4D), and to compare values with fT4 concentration measured directly and with total T4 (TT4) concentration. The fT4D, fT74, and TT4 concentrations were m...

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Bibliographic Details
Published in:Journal of veterinary internal medicine 2006-03, Vol.20 (2), p.371-376
Main Authors: Breuhaus, B.A, Refsal, K.R, Beyerlein, S.L
Format: Article
Language:English
Subjects:
Animals
Assay validation
assays
Dialysis - methods
Dialysis - veterinary
disease course
Equilibrium dialysis
Female
Free thyroxine
Horse
Horse Diseases - blood
Horse Diseases - diagnosis
horses
Horses - blood
hypothyroidism
Hypothyroidism - blood
Hypothyroidism - diagnosis
Hypothyroidism - veterinary
L-thyroxine
Male
Nonthyroidal illness syndrome
normal values
Reference Values
Thyroxine - blood
validity
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Summary:The purpose of the study reported here was to validate measurement of free thyroxine (fT4) concentration in equine serum by equilibrium dialysis (fT4D), and to compare values with fT4 concentration measured directly and with total T4 (TT4) concentration. The fT4D, fT74, and TT4 concentrations were measured over a range of values in euthyroid horses and horses made hypothyroid by administration of propylthiouracil (PTU). Concentrations of fT4D (
ISSN:0891-6640
1939-1676
DOI:10.1111/j.1939-1676.2006.tb02870.x