Measurement of free thyroxine concentration in horses by equilibrium dialysis

The purpose of the study reported here was to validate measurement of free thyroxine (fT4) concentration in equine serum by equilibrium dialysis (fT4D), and to compare values with fT4 concentration measured directly and with total T4 (TT4) concentration. The fT4D, fT74, and TT4 concentrations were m...

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Published in:Journal of veterinary internal medicine 2006-03, Vol.20 (2), p.371-376
Main Authors: Breuhaus, B.A, Refsal, K.R, Beyerlein, S.L
Format: Article
Language:English
Subjects:
Animals
Assay validation
assays
Dialysis - methods
Dialysis - veterinary
disease course
Equilibrium dialysis
Female
Free thyroxine
Horse
Horse Diseases - blood
Horse Diseases - diagnosis
horses
Horses - blood
hypothyroidism
Hypothyroidism - blood
Hypothyroidism - diagnosis
Hypothyroidism - veterinary
L-thyroxine
Male
Nonthyroidal illness syndrome
normal values
Reference Values
Thyroxine - blood
validity
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creator Breuhaus, B.A
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Beyerlein, S.L
description The purpose of the study reported here was to validate measurement of free thyroxine (fT4) concentration in equine serum by equilibrium dialysis (fT4D), and to compare values with fT4 concentration measured directly and with total T4 (TT4) concentration. The fT4D, fT74, and TT4 concentrations were measured over a range of values in euthyroid horses and horses made hypothyroid by administration of propylthiouracil (PTU). Concentrations of fT4D (
doi_str_mv 10.1111/j.1939-1676.2006.tb02870.x
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The fT4D, fT74, and TT4 concentrations were measured over a range of values in euthyroid horses and horses made hypothyroid by administration of propylthiouracil (PTU). Concentrations of fT4D (&lt;1.8–83 pmol/L) were consistently higher than those of fT4 (&lt;1–40 pmol/L). There was a significant (P &lt; .001) regression of fT4D on fT4 in 503 samples from normal horses (y = 2.086x ‐ 0.430). In baseline samples from 71 healthy euthyroid horses, fT4 concentration ranged from 6‐ 21 pmol/L (median, 11 pmol/L; 95% confidence interval [CI]10.5–11.8 pmol/L), and fT4D concentration ranged from 7–47 pmol/L (median, 22 pmol/L; 95% CI 20.9–25.1 pmol/L). Free T4D, fT4, and TT4 concentrations were also measured in 34 ill horses. Horses consuming PTU and ill horses had significantly (P &lt; .05) lower serum concentration of TT4, fT4, and fT4D than did clinically normal, healthy horses. If serum samples from ill horses were further subdivided into samples from horses that lived and samples from horses that died, fT4D concentration was not significantly different in ill horses that lived, compared with that in healthy horses, whereas fT4 concentration was still significantly decreased in ill horses that died (P &lt; 0.001). We conclude that measurement of fT4 concentration by equilibrium dialysis is a valid technique in the horse, and its use may provide improved ability to distinguish nonthyroidal illness syndrome from hypothyroidism in that species.</description><identifier>ISSN: 0891-6640</identifier><identifier>EISSN: 1939-1676</identifier><identifier>DOI: 10.1111/j.1939-1676.2006.tb02870.x</identifier><identifier>PMID: 16594596</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Assay validation ; assays ; Dialysis - methods ; Dialysis - veterinary ; disease course ; Equilibrium dialysis ; Female ; Free thyroxine ; Horse ; Horse Diseases - blood ; Horse Diseases - diagnosis ; horses ; Horses - blood ; hypothyroidism ; Hypothyroidism - blood ; Hypothyroidism - diagnosis ; Hypothyroidism - veterinary ; L-thyroxine ; Male ; Nonthyroidal illness syndrome ; normal values ; Reference Values ; Thyroxine - blood ; validity</subject><ispartof>Journal of veterinary internal medicine, 2006-03, Vol.20 (2), p.371-376</ispartof><rights>2006 American College of Veterinary Internal Medicine</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3461-e2ddd96782d7a9767c7b09e7d86d3831027e39db587c6cab8e515920d42bfc9c3</citedby><cites>FETCH-LOGICAL-c3461-e2ddd96782d7a9767c7b09e7d86d3831027e39db587c6cab8e515920d42bfc9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1939-1676.2006.tb02870.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1939-1676.2006.tb02870.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,11562,27924,27925,46052,46476</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1939-1676.2006.tb02870.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16594596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Breuhaus, B.A</creatorcontrib><creatorcontrib>Refsal, K.R</creatorcontrib><creatorcontrib>Beyerlein, S.L</creatorcontrib><title>Measurement of free thyroxine concentration in horses by equilibrium dialysis</title><title>Journal of veterinary internal medicine</title><addtitle>J Vet Intern Med</addtitle><description>The purpose of the study reported here was to validate measurement of free thyroxine (fT4) concentration in equine serum by equilibrium dialysis (fT4D), and to compare values with fT4 concentration measured directly and with total T4 (TT4) concentration. The fT4D, fT74, and TT4 concentrations were measured over a range of values in euthyroid horses and horses made hypothyroid by administration of propylthiouracil (PTU). Concentrations of fT4D (&lt;1.8–83 pmol/L) were consistently higher than those of fT4 (&lt;1–40 pmol/L). There was a significant (P &lt; .001) regression of fT4D on fT4 in 503 samples from normal horses (y = 2.086x ‐ 0.430). In baseline samples from 71 healthy euthyroid horses, fT4 concentration ranged from 6‐ 21 pmol/L (median, 11 pmol/L; 95% confidence interval [CI]10.5–11.8 pmol/L), and fT4D concentration ranged from 7–47 pmol/L (median, 22 pmol/L; 95% CI 20.9–25.1 pmol/L). Free T4D, fT4, and TT4 concentrations were also measured in 34 ill horses. Horses consuming PTU and ill horses had significantly (P &lt; .05) lower serum concentration of TT4, fT4, and fT4D than did clinically normal, healthy horses. If serum samples from ill horses were further subdivided into samples from horses that lived and samples from horses that died, fT4D concentration was not significantly different in ill horses that lived, compared with that in healthy horses, whereas fT4 concentration was still significantly decreased in ill horses that died (P &lt; 0.001). 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The fT4D, fT74, and TT4 concentrations were measured over a range of values in euthyroid horses and horses made hypothyroid by administration of propylthiouracil (PTU). Concentrations of fT4D (&lt;1.8–83 pmol/L) were consistently higher than those of fT4 (&lt;1–40 pmol/L). There was a significant (P &lt; .001) regression of fT4D on fT4 in 503 samples from normal horses (y = 2.086x ‐ 0.430). In baseline samples from 71 healthy euthyroid horses, fT4 concentration ranged from 6‐ 21 pmol/L (median, 11 pmol/L; 95% confidence interval [CI]10.5–11.8 pmol/L), and fT4D concentration ranged from 7–47 pmol/L (median, 22 pmol/L; 95% CI 20.9–25.1 pmol/L). Free T4D, fT4, and TT4 concentrations were also measured in 34 ill horses. Horses consuming PTU and ill horses had significantly (P &lt; .05) lower serum concentration of TT4, fT4, and fT4D than did clinically normal, healthy horses. If serum samples from ill horses were further subdivided into samples from horses that lived and samples from horses that died, fT4D concentration was not significantly different in ill horses that lived, compared with that in healthy horses, whereas fT4 concentration was still significantly decreased in ill horses that died (P &lt; 0.001). We conclude that measurement of fT4 concentration by equilibrium dialysis is a valid technique in the horse, and its use may provide improved ability to distinguish nonthyroidal illness syndrome from hypothyroidism in that species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16594596</pmid><doi>10.1111/j.1939-1676.2006.tb02870.x</doi><tpages>6</tpages></addata></record>
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identifier ISSN: 0891-6640
ispartof Journal of veterinary internal medicine, 2006-03, Vol.20 (2), p.371-376
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1939-1676
language eng
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source Wiley Online Library Open Access
subjects Animals
Assay validation
assays
Dialysis - methods
Dialysis - veterinary
disease course
Equilibrium dialysis
Female
Free thyroxine
Horse
Horse Diseases - blood
Horse Diseases - diagnosis
horses
Horses - blood
hypothyroidism
Hypothyroidism - blood
Hypothyroidism - diagnosis
Hypothyroidism - veterinary
L-thyroxine
Male
Nonthyroidal illness syndrome
normal values
Reference Values
Thyroxine - blood
validity
title Measurement of free thyroxine concentration in horses by equilibrium dialysis
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