Antibody Response to Aggregated Human Interferon Alpha2b in Wild-type and Transgenic Immune Tolerant Mice Depends on Type and Level of Aggregation

• Published in: Journal of pharmaceutical sciences 2006-05, Vol.95 (5), p.1084-1096
• Main Authors: Hermeling, Suzanne, Schellekens, Huub, Maas, Coen, Gebbink, Martijn F.B.G., Crommelin, Daan J.A., Jiskoot, Wim
• Format: Article
• Language: English
• Subjects: Animals; Antibody Formation - drug effects; Biological and medical sciences; Blotting, Western; Catalysis; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Epitopes; General pharmacology; Humans; Hydrogen-Ion Concentration; Immune Tolerance - immunology; immune tolerant; immunology; interferon alpha2b; Interferon-alpha - chemistry; Interferon-alpha - immunology; Light; Medical sciences; Mice; Mice, Transgenic; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; protein aggregation; protein structure; proteins; Recombinant Proteins; Scattering, Radiation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; transgenic mice
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.20599

The aim of this study was to determine the sensitivity of transgenic immune tolerant mice for the type and level of aggregation of recombinant human interferon alpha2b (rhIFNα2b). RhIFNα2b was aggregated by metal-catalyzed oxidation or by incubation at elevated temperature and various pHs. Native rhIFNα2b was mixed with oxidized rhIFNα2b at different ratios to obtain samples with different aggregation levels. The preparations were characterized by UV and fluorescence spectroscopy, gel permeation chromatography (GPC), dynamic light scattering (DLS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and Western blotting, and ELISA. The immunogenicity was evaluated in wild-type mice and transgenic mice immune tolerant for hIFNα2. Sera were analyzed by ELISA for the presence of rhIFNα2b-specific antibodies. The oxidized and aged preparations widely differed regarding the level and nature of aggregates. All preparations containing aggregates increased the immune response in the wild-type mice as compared to native rhIFNα2b and were able to break the tolerance of the transgenic mice. The more native-like the conformation of the aggregated proteins, the more immunogenic the preparations were in the transgenic mice. The native-like aggregates prepared via metal catalysis induced a dose-dependent loss of tolerance in the transgenic mice. In conclusion, the transgenic mouse model can be used to screen rhIFNα2b formulations for low levels of immunogenic aggregates obtained under accelerated storage conditions.