African green monkey kidney Vero cells require de novo protein synthesis for efficient herpes simplex virus 1-dependent apoptosis

During HSV-1 infection, IE gene expression triggers apoptosis, but subsequent synthesis of infected cell proteins blocks apoptotic death from ensuing. This “HSV-1-dependent” apoptosis was identified in HEp-2/HeLa cells infected with wild-type HSV-1 in the presence of an inhibitor of protein synthesi...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2005-06, Vol.336 (2), p.274-290
Main Authors: Nguyen, Marie L., Kraft, Rachel M., Blaho, John A.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cell Line, Tumor
Cercopithecus aethiops
HEp-2/HeLa
Herpes simplex virus
Herpes simplex virus 1
Herpesvirus 1, Human - physiology
Humans
Immediate-Early Proteins - biosynthesis
Membrane Potentials
Mitochondria - physiology
Poly(ADP-ribose) Polymerases - metabolism
Protein synthesis
Species Specificity
Time Factors
Vero
Vero Cells - physiology
Vero Cells - virology
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Summary:During HSV-1 infection, IE gene expression triggers apoptosis, but subsequent synthesis of infected cell proteins blocks apoptotic death from ensuing. This “HSV-1-dependent” apoptosis was identified in HEp-2/HeLa cells infected with wild-type HSV-1 in the presence of an inhibitor of protein synthesis or a virus lacking ICP27 {HSV-1(vBSΔ27)}. Unlike HEp-2/HeLa cells, vBSΔ27-infected Vero cells fail to exhibit dramatic apoptotic morphologies at times prior to 24 hpi. Here, we examined the basis of these different apoptotic responses to HSV-1. We found that infected Vero cells take substantially longer than HEp-2/HeLa cells to display membrane blebbing, chromatin condensation, DNA laddering, and PARP cleavage. Vero, but not HEp-2/HeLa, cells required de novo protein synthesis to exhibit efficient HSV-1-dependent apoptosis, which included changes in mitochondrial membrane potential, and these factors were produced prior to 3 hpi. Vero cells infected with recombinant viruses devoid of the ICP27 and ICP4 proteins alone or both the ICP27 and ICP22 proteins were apoptotic. These results indicate a requirement for cellular or other viral protein synthesis in Vero cells and provide insight into cell type differences in HSV-1-dependent apoptosis.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2005.03.026