Construction of novel human monoclonal antibodies neutralizing Porphyromonas gingivalis hemagglutination activity using transgenic mice expressing human Ig loci

Porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis, and its colonization of subgingival sites is critical in the pathogenic process. One potential virulence factor, hemagglutinin, may mediate bacteria attachment onto and penetration into h...

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Bibliographic Details
Published in:Vaccine 2005-05, Vol.23 (29), p.3850-3856
Main Authors: Shibata, Yasuko, Hosogi, Yumiko, Hayakawa, Mitsuo, Hori, Nobuaki, Kamada, Masafumi, Abiko, Yoshimitsu
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Applied microbiology
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hemagglutination Tests
Hemagglutinin
Hemagglutinins - genetics
Hemagglutinins - immunology
Human monoclonal antibody
Humans
Hybridomas
Mice
Mice, Transgenic
Microbiology
Neutralization Tests
Periodontitis - microbiology
Periodontitis - prevention & control
Porphyromonas gingivalis
Porphyromonas gingivalis - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis, and its colonization of subgingival sites is critical in the pathogenic process. One potential virulence factor, hemagglutinin, may mediate bacteria attachment onto and penetration into host cells, as well as agglutinate and lyses erythrocytes to intake heme, an absolute requirement for growth. Toward the development of passive immunotherapy, the construction of a human type monoclonal antibody, which is capable of inhibiting the hemagglutinating ability, will be significant and important. The human mAbs, both exhibiting a high degree of specificity and affinity against the recombinant 130 kDa hemagglutinin domain protein have been prepared using XenoMouse technology. The constructed Xeno-mAbs, IgG2 subclass, significantly inhibited hemagglutination of P. gingivalis and its vesicles. The newly constructed Xeno-mAbs may prove to be useful for the development of passive immunization against periodontal diseases caused by P. gingivalis infection, pending the results of fertility study in disease mode.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2005.01.159