Long-term reinnervation effects after sciatic nerve lesions in adult rats

• Published in: Annals of anatomy 2005-04, Vol.187 (2), p.113-120
• Main Authors: IJkema-Paassen, Jos, Meek, Marcel F., Gramsbergen, Albert
• Format: Article
• Language: English
• Subjects: Animals; Female; Fiber-type distribution; Long term; Male; Muscle Denervation; Muscle, Skeletal - innervation; Nerve Fibers - physiology; Nerve Regeneration; Polyneural innervation; Rat; Rats; Reinnervation; Sciatic Nerve - anatomy & histology; Sciatic Nerve - physiology; Transplantation, Autologous
• ISSN: 0940-9602; 1618-0402
• DOI: 10.1016/j.aanat.2004.11.001

Transection of the sciatic nerve in adult rats induces drastic changes in hindleg muscles. Earlier, we demonstrated that the reinnervated soleus (SOL) muscle, 21 weeks after a transection mainly contains type II fibers. This is in striking contrast to normal muscle, which consists to 80% of type I muscle fibers. Also we observed 13.9% of the fibers to be polyneurally innervated. The problem of the present study is whether these changes are reversible after longer survival periods. Therefore, the SOL was studied 60 weeks after transection and reconstruction by an autologous nerve graft. In six rats, we studied muscle fiber distributions by monoclonal antibodies, and innervation patterns by cholinesterase staining and AgNO 3 impregnation. Still at 60 weeks, only 20% of the muscle fibers are of type I and this is similar to results at 21 weeks, indicating that no recovery to the normal has been reached by that age. Furthermore, 20% of the endplates in the reinnervated SOL were polyneurally innervated, but we also observed this in 10% of the endplates on the control side. These increases, compared to data at 21 weeks, are interpreted as an aging effect.