Determination of the 5'- and 3'-terminal sequences completes the sequences of the two double-stranded RNAs of Penicillium stoloniferum virus S

The two genomic segments of Penicillium Stoloniferum virus S (PsV-S), a member of the Partitiviridae, were recently sequenced and published. We independantly sequenced PsV-S and showed that the original sequence was missing nucleotides at both the 5' and 3' termini of both segments. We det...

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Bibliographic Details
Published in:Virus genes 2006-04, Vol.32 (2), p.137-138
Main Authors: Hacker, Caroline V, Brasier, Clive M, Buck, Kenneth W
Format: Article
Language:English
Subjects:
Base Sequence
Capsid Proteins - genetics
Genome, Viral
Molecular Sequence Data
Partitiviridae
Penicillium - virology
Penicillium stoloniferum virus S
RNA Replicase - genetics
RNA Viruses - genetics
RNA, Viral - genetics
Sequence Analysis, RNA
Sequence Homology, Nucleic Acid
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Summary:The two genomic segments of Penicillium Stoloniferum virus S (PsV-S), a member of the Partitiviridae, were recently sequenced and published. We independantly sequenced PsV-S and showed that the original sequence was missing nucleotides at both the 5' and 3' termini of both segments. We determined the correct sequence in three independent experiments and found the segments to be 1753 bp (encoding the RNA-dependant RNA polymerase) and 1581 bp (encoding the Capsid Protein). Homology was shown between the 5' and 3' ends of PsV-S and other members of the Partitiviridae.
ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-005-6868-2