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Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response
We examined Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to M. p...
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| Published in: | FEMS immunology and medical microbiology 2005-06, Vol.44 (3), p.277-282 |
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| Main Authors: | , , , , , , |
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| container_end_page | 282 |
| container_issue | 3 |
| container_start_page | 277 |
| container_title | FEMS immunology and medical microbiology |
| container_volume | 44 |
| creator | Lo, Shyh-Ching Wang, Richard Y-H. Grandinetti, Teresa Zou, Nianxiang Hayes, Michael M. Shih, Jim W-K. Wear, Douglas J. |
| description | We examined
Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom
M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to
M. penetrans isolation reacted with a number of
M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of
M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to
M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of
M. penetrans with molecular mass of 35 and 38
kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for
M. penetrans infection. |
| doi_str_mv | 10.1016/j.femsim.2004.12.010 |
| format | article |
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Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom
M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to
M. penetrans isolation reacted with a number of
M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of
M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to
M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of
M. penetrans with molecular mass of 35 and 38
kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for
M. penetrans infection.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1016/j.femsim.2004.12.010</identifier><identifier>PMID: 15907449</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Acquired immune deficiency syndrome ; AIDS ; AIDS-Related Opportunistic Infections - blood ; Antibodies ; Antibodies, Bacterial - blood ; Antibody response ; Antibody Specificity ; Antigens ; Antigens, Bacterial - analysis ; Antigens, Bacterial - immunology ; Bacterial Outer Membrane Proteins - analysis ; Bacteriology ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Human viral diseases ; Humans ; Immunoblotting ; Infectious diseases ; Lamps ; Lipids ; Major antigen ; Male ; Medical sciences ; Membrane proteins ; Microbiology ; Miscellaneous ; Mycoplasma Infections - blood ; Mycoplasma penetrans ; Mycoplasma penetrans - immunology ; Mycoplasma penetrans - isolation & purification ; Pattern recognition ; Proteins ; Seroconversion ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids</subject><ispartof>FEMS immunology and medical microbiology, 2005-06, Vol.44 (3), p.277-282</ispartof><rights>2005 Federation of European Microbiological Societies</rights><rights>2005 Federation of European Microbiological Societies. 2005</rights><rights>2005 INIST-CNRS</rights><rights>2005 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5387-661ab685f56cf3d3368fd79918b4f2e4f82e9008866f2653f765955f659f8c7a3</citedby><cites>FETCH-LOGICAL-c5387-661ab685f56cf3d3368fd79918b4f2e4f82e9008866f2653f765955f659f8c7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16800612$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15907449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lo, Shyh-Ching</creatorcontrib><creatorcontrib>Wang, Richard Y-H.</creatorcontrib><creatorcontrib>Grandinetti, Teresa</creatorcontrib><creatorcontrib>Zou, Nianxiang</creatorcontrib><creatorcontrib>Hayes, Michael M.</creatorcontrib><creatorcontrib>Shih, Jim W-K.</creatorcontrib><creatorcontrib>Wear, Douglas J.</creatorcontrib><title>Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>We examined
Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom
M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to
M. penetrans isolation reacted with a number of
M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of
M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to
M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of
M. penetrans with molecular mass of 35 and 38
kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for
M. penetrans infection.</description><subject>Acquired immune deficiency syndrome</subject><subject>AIDS</subject><subject>AIDS-Related Opportunistic Infections - blood</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibody response</subject><subject>Antibody Specificity</subject><subject>Antigens</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Outer Membrane Proteins - analysis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Infectious diseases</subject><subject>Lamps</subject><subject>Lipids</subject><subject>Major antigen</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane proteins</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycoplasma Infections - blood</subject><subject>Mycoplasma penetrans</subject><subject>Mycoplasma penetrans - immunology</subject><subject>Mycoplasma penetrans - isolation & purification</subject><subject>Pattern recognition</subject><subject>Proteins</subject><subject>Seroconversion</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNks1u1DAUhSMEokPhDRCyhGA3U9uJf8ICqSoURmrFApDYWR7nuvUoiVM70yqPwttyh4yoxAK6sS37O-f6-rgoXjK6YpTJk-3KQ5dDt-KUVivGV5TRR8WCCVUtZS1-PC4WtOZ6qXlVHRXPct5SBGtKnxZHTNRUVVW9KH5eTi4Orc2dJQP0MCbbZxJ6D24MEZe2b0iGFF3sbyFl3MNTMtgxQD9mchfGa3K6_vD1HQkN7gQfnN0rSfSks9uYSPenQotuY7gCtB1tuoIRGrKZyHXM4--TTWwmkiAPWBieF0-8bTO8OMzHxffzj9_OPi8vvnxan51eLJ0otVpKyexGauGFdL5sylJq36i6ZnpTeQ6V1xywaa2l9FyK0ispaiE8jl47Zcvj4u3sO6R4s4M8mi5kB21re4i7bKTSguqS_xdkSlCOD4zg67_AbdylHpswvKSKCcGoQKqaKZdizgm8GVLobJoMo2afsNmaOWGzT9gwbjBhlL06mO82HTT3okOkCLw5ADY723oM1IV8z0lNqWT7dvTM3YUWpgcVN-frS64USk9madwND731-1kBmORtgGSyww_koAkJf5ppYvi3wS-34ek5</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>Lo, Shyh-Ching</creator><creator>Wang, Richard Y-H.</creator><creator>Grandinetti, Teresa</creator><creator>Zou, Nianxiang</creator><creator>Hayes, Michael M.</creator><creator>Shih, Jim W-K.</creator><creator>Wear, Douglas J.</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7QL</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200506</creationdate><title>Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response</title><author>Lo, Shyh-Ching ; Wang, Richard Y-H. ; Grandinetti, Teresa ; Zou, Nianxiang ; Hayes, Michael M. ; Shih, Jim W-K. ; Wear, Douglas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5387-661ab685f56cf3d3368fd79918b4f2e4f82e9008866f2653f765955f659f8c7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>AIDS</topic><topic>AIDS-Related Opportunistic Infections - blood</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibody response</topic><topic>Antibody Specificity</topic><topic>Antigens</topic><topic>Antigens, Bacterial - analysis</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial Outer Membrane Proteins - analysis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Infectious diseases</topic><topic>Lamps</topic><topic>Lipids</topic><topic>Major antigen</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane proteins</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycoplasma Infections - blood</topic><topic>Mycoplasma penetrans</topic><topic>Mycoplasma penetrans - immunology</topic><topic>Mycoplasma penetrans - isolation & purification</topic><topic>Pattern recognition</topic><topic>Proteins</topic><topic>Seroconversion</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lo, Shyh-Ching</creatorcontrib><creatorcontrib>Wang, Richard Y-H.</creatorcontrib><creatorcontrib>Grandinetti, Teresa</creatorcontrib><creatorcontrib>Zou, Nianxiang</creatorcontrib><creatorcontrib>Hayes, Michael M.</creatorcontrib><creatorcontrib>Shih, Jim W-K.</creatorcontrib><creatorcontrib>Wear, Douglas J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lo, Shyh-Ching</au><au>Wang, Richard Y-H.</au><au>Grandinetti, Teresa</au><au>Zou, Nianxiang</au><au>Hayes, Michael M.</au><au>Shih, Jim W-K.</au><au>Wear, Douglas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2005-06</date><risdate>2005</risdate><volume>44</volume><issue>3</issue><spage>277</spage><epage>282</epage><pages>277-282</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>We examined
Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom
M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole cell proteins. Serum samples obtained prior to
M. penetrans isolation reacted with a number of
M. penetrans proteins, most likely due to non-specific cross-reactions. Further analysis revealed that patients produced prominent antibody reaction to lipid-associated membrane proteins (LAMPs) of
M. penetrans at the time of mycoplasma isolation, which could not be observed for serum samples obtained prior to
M. penetrans isolation. The positive antibody reaction was mainly directed against two major LAMPs of
M. penetrans with molecular mass of 35 and 38
kDa and produced a distinctive pattern of positive immunoreaction bands. Our observation suggested that, comparing with whole mycoplasmal proteins, LAMPs were more specific target antigens in serological assays for
M. penetrans infection.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>15907449</pmid><doi>10.1016/j.femsim.2004.12.010</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0928-8244 |
| ispartof | FEMS immunology and medical microbiology, 2005-06, Vol.44 (3), p.277-282 |
| issn | 0928-8244 1574-695X 2049-632X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67850832 |
| source | Oxford Journals Online |
| subjects | Acquired immune deficiency syndrome AIDS AIDS-Related Opportunistic Infections - blood Antibodies Antibodies, Bacterial - blood Antibody response Antibody Specificity Antigens Antigens, Bacterial - analysis Antigens, Bacterial - immunology Bacterial Outer Membrane Proteins - analysis Bacteriology Biological and medical sciences Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Human viral diseases Humans Immunoblotting Infectious diseases Lamps Lipids Major antigen Male Medical sciences Membrane proteins Microbiology Miscellaneous Mycoplasma Infections - blood Mycoplasma penetrans Mycoplasma penetrans - immunology Mycoplasma penetrans - isolation & purification Pattern recognition Proteins Seroconversion Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids |
| title | Mycoplasma penetrans infections and seroconversion in patients with AIDS: identification of major mycoplasmal antigens targeted by host antibody response |
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