Genome‐wide identification of genes required for growth of Saccharomyces cerevisiae under ethanol stress

The Saccharomyces cerevisiae deletion collection was screened for impaired growth on glucose‐based complex medium containing 6% ethanol. Forty‐six mutants were found. Genes encoding proteins involved in vacuolar function, the cell integrity pathway, mitochondrial function, subunits of the co‐chapero...

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Published in:Yeast (Chichester, England) England), 2006-04, Vol.23 (5), p.351-359
Main Authors: van Voorst, Frank, Houghton‐Larsen, Jens, Jønson, Lars, Kielland‐Brandt, Morten C., Brandt, Anders
Format: Article
Language:English
Subjects:
Beer
Blotting, Western
Calcofluor white
cell integrity
DNA, Fungal - genetics
DNA, Fungal - metabolism
Ethanol - pharmacology
ethanol sensitivity
Fermentation
Fungal Proteins - genetics
Fungal Proteins - physiology
Genes, Fungal - drug effects
Genes, Fungal - physiology
Genome, Fungal
GFP
high‐gravity fermentation
Mutation
Polymerase Chain Reaction
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Signal Transduction
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Summary:The Saccharomyces cerevisiae deletion collection was screened for impaired growth on glucose‐based complex medium containing 6% ethanol. Forty‐six mutants were found. Genes encoding proteins involved in vacuolar function, the cell integrity pathway, mitochondrial function, subunits of the co‐chaperone complex GimC and components of the SAGA transcription factor complex were in this way found to be important for the growth of wild‐type Saccharomyces yeast in the presence of ethanol. Several mutants were also sensitive to Calcofluor white (14 mutants), sorbic acid (9), increased temperature (5) and NaCl (3). The transcription factors Msn2p and Ars1p, tagged with green fluorescent protein, were translocated to the nucleus upon ethanol stress. Only one of the genes that contain STRE elements in the promoter was important under ethanol stress; this was TPS1, encoding trehalose 6‐phosphate synthase. The map kinase of the cell integrity pathway, Slt2p, was phosphorylated when cells were treated with 6% ethanol. Two out of three mutants tested fermented 20% glucose more slowly than the wild‐type. Copyright © 2006 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1359