Role of Staphylococcus aureus global regulators sae and sigmaB in virulence gene expression during device-related infection

The ability of Staphylococcus aureus to adapt to different environments is due to a regulatory network comprising several loci. Here we present a detailed study of the interaction between the two global regulators sae and sigmaB of S. aureus and their influence on virulence gene expression in vitro,...

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Bibliographic Details
Published in:Infection and immunity 2005-06, Vol.73 (6), p.3415
Main Authors: Goerke, Christiane, Fluckiger, Ursula, Steinhuber, Andrea, Bisanzio, Vittoria, Ulrich, Martina, Bischoff, Markus, Patti, Joseph M, Wolz, Christiane
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Base Sequence
Gene Expression Regulation, Bacterial
Guinea Pigs
Molecular Sequence Data
Prostheses and Implants - adverse effects
RNA, Messenger - analysis
Sigma Factor - genetics
Sigma Factor - physiology
Staphylococcus aureus - genetics
Staphylococcus aureus - growth & development
Staphylococcus aureus - pathogenicity
Transcription Factors
Virulence
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Summary:The ability of Staphylococcus aureus to adapt to different environments is due to a regulatory network comprising several loci. Here we present a detailed study of the interaction between the two global regulators sae and sigmaB of S. aureus and their influence on virulence gene expression in vitro, as well as during device-related infection. The expression of sae, asp23, hla, clfA, coa, and fnbA was determined in strain Newman and its isogenic saeS/R and sigB mutants by Northern analysis and LightCycler reverse transcription-PCR. There was no indication of direct cross talk between the two regulators. sae had a dominant effect on target gene expression during device-related infection. SigmaB seemed to be less active throughout the infection than under induced conditions in vitro.
ISSN:0019-9567
DOI:10.1128/iai.73.6.3415-3421.2005