Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo

In this study, we isolated CD31 −, CD34 −, CD106 − (VCAM-1 −), and fetal liver kinase + (Flk1 +) cells from adipose tissue. These cells can be induced to differentiate into cells of osteogenic and adipogenic lineages in vitro and were termed adipose derived adult stem cells (ADAS cells). We also sho...

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Published in:Biochemical and biophysical research communications 2005-07, Vol.332 (2), p.370-379
Main Authors: Cao, Ying, Sun, Zhao, Liao, Lianming, Meng, Yan, Han, Qin, Zhao, Robert Chunhua
Format: Article
Language:English
Subjects:
Adipocytes - pathology
Adipocytes - transplantation
Adipose
Animals
Cell Culture Techniques - methods
Cell Differentiation
Cell Proliferation
Cell Size
Cells, Cultured
Differentiation
Endothelial
Endothelial Cells - pathology
Humans
Inhibitor
Ischemia
Ischemia - pathology
Ischemia - surgery
Mesenchymal Stromal Cells - pathology
Mice
Mice, Nude
Neovascularization, Physiologic
Stem cells
Tissue Engineering - methods
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Summary:In this study, we isolated CD31 −, CD34 −, CD106 − (VCAM-1 −), and fetal liver kinase + (Flk1 +) cells from adipose tissue. These cells can be induced to differentiate into cells of osteogenic and adipogenic lineages in vitro and were termed adipose derived adult stem cells (ADAS cells). We also showed that they have characteristics of endothelial progenitor cells. In vitro, ADAS cells expressed endothelial markers when cultured with VEGF. In vivo, ADAS cells can differentiate in response to local cues into endothelial cells that contributed to neoangiogenesis in hindlimb ischemia models. PI3 kinase inhibitor LY294002 blocked the differentiation of ADAS cells into endothelial cells in vitro. Because ADAS cells can be expanded in culture without obvious senescence for more than 20 population doublings, they may be a potential source of endothelial cells for cellular pro-angiogenic therapies.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.04.135